IN VITRO STUDY OF THE GROWTH, REPRODUCTION, ANTIBIOTIC RESISTANCE, AND COMPETITIVE RELATIONSHIPS OF A LACTOBACILLUS CASEI RHAMNOSUS STRAIN
- Authors: Savicheva A.M.1, Rybina E.V.1
-
Affiliations:
- D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Sciences
- Issue: No 7 (2014)
- Pages: 79-83
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247262
- ID: 247262
Cite item
Open Access
Access granted
Abstract
Objective. To determine the number of Lactobacillus casei rhamnosus entering the composition of lactoginal (BESINS Healthcare, Belgium) and the pH value of the culture medium during regeneration of these lactobacilli and to study their antibiotic resistance and competitive relationships with yeast-like fungi of the Candida genus. Subject and methods. The lyophilized cultures of Lactobacillus casei rhamnosus and 10 clinical yeast-like Candida albicans fungus strains, 2 C. krusei strains, and 3 C. glabrata strains were investigated. The pH of the liquid MRS medium was measured during L. c. rhamnosus regeneration during the first 24 hours. The antibiotic susceptibility of lactobacilli was determined by the disk-diffusion method. The inhibitory properties of L. с. rhamnosus against yeast-like Candida fungi were determined during 24-72 hours after concomitantly culturing lactobacilli and yeast-like fungi. Results. The probiotic lactoginal contains live L. c. rhamnosus bacteria at a concentration of 10 11 CFU/ml, which are able within the first 24 hours to decrease pH and to suppress the growth of yeast-like fungi resistant to antibiotics that are most commonly used in obstetrics and gynecology. Conclusion. Lactoginal may be used to restore impaired vaginal microbiocenosis in both nonpregnant and pregnant women in any pregnancy periods.
Full Text
About the authors
Alevtina Mikhailovna Savicheva
D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Sciences
Email: savitcheva@mail.ru
MD, professor, head of Laboratory of Microbiology 199034, Russia, St. Petersburg, Mendeleyevskaya Line 3
Elena Vladimirovna Rybina
D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Sciences
Email: elena.rybina@gmail.com
bacteriologist, laboratory of microbiology 199034, Russia, St. Petersburg, Mendeleyevskaya Line 3
References
- Joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of a Joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. 2002; 34 p.
- Evrard B., Coudeyras S., Dosgilbert A., Charbonnel N., Alame'J., Tridon A., Forestier C. Dose-dependent immunomodulation of human dendritic cells by the probiotic Lactobacillus rhamnosus Lcr35. PLoS One. 2011; 6(4): e18735.
- Ding W.K., Shah N.P. Effect of various encapsulating materials on the stability of probiotic bacteria. J. Food Sci. 2009; 74(2): 100-7.
- Jacobsen C.N., Rosenfeldt Nielsen V., Hayford A.E., M0ller P.L., Michaelsen K.F. et al. Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl. Environ. Microbiol. 1999; 65(11): 4949-56.
- Lepargneur J.-P., Rousseau V. Rôle protecteur de la flore de Doderleïn. J. Gynecol. Obstet. Biol. Reprod. 2002; 31(5): 485-94.
- Saarela M., Virkajärvi I., Alakomi H.L., Mattila-Sandholm T., Vaari A., Suomalainen T., Mättö J. Influence of fermentation time, cryoprotectant and neutralization of cell concentrate on freeze-drying survival, storage stability, and acid and bile exposure of Bifidobacterium animalis ssp. lactis cells produced without milk-based ingredients. J. Appl. Microbiol. 2005; 99(6) :1330-9.
- Grzeskowiak L., Isolauri E., Salminen S., Gueimonde M. Manufacturing process influences properties of probiotic bacteria. Br. J. Nutr. 2011; 105(6): 887-94.
- Coudeyras S., Jugie G., Vermerie M., Forestier C. Adhesion of human probiotic Lactobacillus rhamnosus to cervical and vaginal cells and interaction with vaginosis-associated pathogens. Infect. Dis. Obstet. Gynecol. 2008; 2008: 549640.
- Coudeyras S., Marchandin H., Fajon C., Forestier C. Taxonomic and strain-specific identification of the probiotic strain Lactobacillus rhamnosus 35 within the Lactobacillus casei group. Appl. Environ. Microbiol. 2008; 74(9): 2679-2689.
- Forestier C., De Champs C., Vatoux C., Joly B. Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties. Res. Microbiol. 2001; 152(2): 167-73.
- De Champs C., Maroncle N., Balestrino D., Rich C., Forestier C. Persistence of colonization of intestinal mucosa by a probiotic strain Lactobacillus casei subsp. rhamnosus Lcr35, after oral consumption. J. Clin. Microbiol. 2003; 41(3): 1270-3.
- Определение чувствительности микроорганизмов к антибактериальным препаратам: Методические указания МУК 4.2.1890-04. Клиническая микробиология и антимикробная химиотерапия. 2004; 6(4): 306-59.
- The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 4.0. 2014; 79 p.
- Disc diffusion supplemental tables. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute; 2007; 34-161.
- Falagas M. E., Betsi G. I., Athanasiou S. Probiotics for the treatment of women with bacterial vaginosis. Clin. Microbiol. Infect. 2007; 13(7): 657-64.
- Kern A.M., Bohbot J.M., Cardot J.M. Preventive treatment of vulvovaginal candidiasis with a vaginal probiotics (Gynophilus® -Lcr Regenerans®): results of the Candiflore observational study. La Lettre du Gynécologue. 2012; no. 370.
- Atassi F., Brassart D., Grob P., Graf F., Servin A.L. Lactobacillus strains isolated from the vaginal microbiota of healthy women inhibit Prevotella bivia and Gardnerella vaginalis in coculture and cell culture. FEMS Immunol. Med. Microbiol. 2006; 48(3): 424-32.
- Strus M., Kucharska A., Kukla G., Brzychczy-Wloch M., Maresz K., Heczko P.B. The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida. Infect. Dis. Obstet. Gynecol. 2005; 13(2): 69-75.
- Petricevic L., Witt A. The role of Lactobacillus casei rhamnosus Lcr35 in restoring the normal vaginal flora after antibiotic treatment of bacterial vaginosis. Br. J. Obstet. Gynaecol. 2008; 115(11): 1369-74.
Supplementary files
