IN VITRO STUDY OF THE GROWTH, REPRODUCTION, ANTIBIOTIC RESISTANCE, AND COMPETITIVE RELATIONSHIPS OF A LACTOBACILLUS CASEI RHAMNOSUS STRAIN


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Abstract

Objective. To determine the number of Lactobacillus casei rhamnosus entering the composition of lactoginal (BESINS Healthcare, Belgium) and the pH value of the culture medium during regeneration of these lactobacilli and to study their antibiotic resistance and competitive relationships with yeast-like fungi of the Candida genus. Subject and methods. The lyophilized cultures of Lactobacillus casei rhamnosus and 10 clinical yeast-like Candida albicans fungus strains, 2 C. krusei strains, and 3 C. glabrata strains were investigated. The pH of the liquid MRS medium was measured during L. c. rhamnosus regeneration during the first 24 hours. The antibiotic susceptibility of lactobacilli was determined by the disk-diffusion method. The inhibitory properties of L. с. rhamnosus against yeast-like Candida fungi were determined during 24-72 hours after concomitantly culturing lactobacilli and yeast-like fungi. Results. The probiotic lactoginal contains live L. c. rhamnosus bacteria at a concentration of 10 11 CFU/ml, which are able within the first 24 hours to decrease pH and to suppress the growth of yeast-like fungi resistant to antibiotics that are most commonly used in obstetrics and gynecology. Conclusion. Lactoginal may be used to restore impaired vaginal microbiocenosis in both nonpregnant and pregnant women in any pregnancy periods.

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About the authors

Alevtina Mikhailovna Savicheva

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Sciences

Email: savitcheva@mail.ru
MD, professor, head of Laboratory of Microbiology 199034, Russia, St. Petersburg, Mendeleyevskaya Line 3

Elena Vladimirovna Rybina

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Sciences

Email: elena.rybina@gmail.com
bacteriologist, laboratory of microbiology 199034, Russia, St. Petersburg, Mendeleyevskaya Line 3

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