The specific features of cellular immunity in pregnant women with systemic lupus erythematosus and their newborn infants


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Abstract

Subjects and methods. This prospective study enrolled 34 pregnant women with grade I-IIISLE and their neonates (a study group). The women took cytostatics and corticosteroids during pregnancy. A control group consisted of 21 women with the physiological course of pregnancy and labor, without autoimmune diseases, as well as their neonates. The count of main lymphocyte subpopulations and that of regulatory T cells CD4 +CD25 hlghCD127 low/ ) were measured in the maternal peripheral blood obtained at the end of the third trimester before labor and in the neonatal umbilical cord blood. The lymphocytes were phenotyped by flow cytometry using fluorescently labeled monoclonal antibodies to differentiated lymphocyte antigens. Results. There were considerable changes in the subpopulation composition of peripheral blood lymphocytes in pregnant women with SLE, which manifested themselves as an increase in the count of activated T lymphocytes (CD3 +HLA-DR +) and regulatory T cells (CD4 +CD25 hlgl/CD127 low/ ) and a decrease in that of natural cytotoxic cells (CD56/16 +). There were no significant differences in the subpopulation composition of neonatal umbilical cord blood lymphocytes in the study and control groups. Conclusion. During pregnancy, the cellular bases of fetal adaptive immunity may normally form and develop in pregnant women with SLE running during therapy with glucocorticosteroids and cytostatics.

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About the authors

N. K Matveeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: matveeva_nk@mail.ru
candidate of biological science, senior researcher of clinical immunology laboratory

L. V Vanko

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: lvanko@mail.ru
MD, professor, leading research worker of clinical immunology laboratory

E. V Fedorova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: KatrinaFedorova7@gmail.com
postgraduate of the 1st obstetric pregnancy pathology department

A. S Belyaeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: skazka_morozko@mail.ru
junior research worker of clinical immunology laboratory

N. I Klimenchenko

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: natalite@list.ru
PhD, head of the 1st obstetric pregnancy pathology department

M. A Nikolaeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
doctor of biological science, leading researcher of clinical immunology laboratory

V. G Safronova

Institute of Cell Biophysics, Russian Academy of Sciences

Email: safronova@icb.psn.ru
candidate of biological science, head of cellular neurobiology laboratory, Institute of Cell Biophysics

N. M Kosheleva

Academician V.A. Nasonova Research Institute of Rheumatology, Russian Academy of Medical Sciences

Email: nadkosheleva@yandex.ru
PhD, senior researcher of the 4th rheumatologic department

L. V Krechetova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
hD, head of clinical immunology laboratory

G. T Sukhikh

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
MD, professor

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