Placental apoptosis and antioxidant defense enzyme gene expression in preeclampsia


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Abstract

Objective: to determine the level of placental apoptosis and antioxidant defense enzyme genes (glutathione peroxidase (GPX1) and glutathione reductase (GSR)) expression in preeclampsia. Subjects and methods. The investigation included 31 patients. All the patients were divided into 2 groups: 1) 11 pregnant women with preeclampsia (a study group); 2) 20 healthy individuals (a comparison group). The gene expression was studied by a qPCR assay and apoptosis was evaluated by the TUNEL method in the placenta. Results. In preeclampsia, the placental expression of glutathione reductase and GPX1 was ascertained to be increased in 100 and 40% of cases, respectively. In the preeclamptic patients, the level of placental apoptosis was also found to be considerably higher than that in apparently healthy individuals and in some cases stem villi were destroyed due to programmed cell death in the villous stroma and syncyotiotrophoblast. Conclusion. GPX1 and GSD gene expression increases in the placenta of preeclamptic patients. Oxidative stress gives rise to the higher level of apoptosis in the placental villi. Not only trophoblast cells, but also, in some cases, villous stromal cells may undergo apoptosis.

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About the authors

Gennady T. Sukhikh

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
PhD, MD, professor, academician of RAS, Director

Aleksey M. Krasnyi

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_krasnyi@oparina4.ru
PhD, the head of the cytology laboratory

Natalia E. Kan

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_kan@oparina4.ru
PhD, MD, the chief physician

Tatiana D. Maiorova

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: t_mayorova@oparina4.ru
PhD, senior scientific researcher of the cytology laboratory

Victor L. Tyutyunnik

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_tioutiounnik@oparina4.ru
PhD, MD, the head of the obstetric observation department

Petimat A. Khovkhaeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: p_hovhaeva@oparina4.ru
the postgraduate student

Olga A. Sergunina

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_sergunina@oparina4.ru
obstetrician-gynecologist of the obstetric observation department

Nataliya V. Tyutyunnik

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tysia07@bk.ru
the resident

Maria I. Gracheva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: m_gracheva@oparina4.ru
obstetrician-gynecologist of the obstetric observation department

Olga V. Vavina

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_vavina@oparina4.ru
the postgraduate student

Nikolay D. Ozernyuk

N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences

Email: ozernyuk@mail.ru
PhD, MD, professor, Director

Daiana A. Boris

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia

Email: dayana_boris@mail.ru
6 year student

References

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