Trend in the production of anti-leukocyte antibodies against paternal antigens during allogeneic cell immunization in women with recurrent miscarriage


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Abstract

Objective. To estimate the trend in the production of anti-paternal anti-leukocyte antibodies during partner cell immunization of women with recurrent miscarriage in the pregestational preparation period and in the f irst trimester of pregnancy. Subjects and methods. Flow cytometry was used to determine anti-paternal antibodies (anti-PAbs) in 53 women with recurrent miscarriage, 20 fertile nonpregnant women, and 7 women with physiological primipregnancy. Results. In the first trimester, no anti-PAbs were found in the control group of primigravida. There were no differences in anti-PAb levels in the women with recurrent miscarriage before treatment and in the fertile women. An immunocytotherapy procedure resulted in a gradual significant increase in anti-PAbs as compared to the baseline level. In the patients who had become pregnant, the level of anti-PAbs remained high during the first trimester. Conclusion. The findings are suggestive of the importance of the anti-PAb test as reflecting the immunomodulatory effect of allogeneic partner cell administration

About the authors

Lyubov V. Krechetova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology

Nelly A. Khachatryan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: nelly1986@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Nana K. Tetruashvili

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: n_tetruashvili@oparina4.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy

Valentina V. Vtorushina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Elena Olegovna Stepanova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: elena2404.07@mail.ru
Junior researcher, Laboratory of Clinical Immunology

Elena L. Golubeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: e_golubeva@oparina4.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Marina A. Nikolaeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology

Gennady T. Sukhikh

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
PhD, MD, professor, academician of RAS, Director

References

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