IMPAIRED EXPRESSION OF THE PROTEINS MGMT AND EZRIN IN ENDOMETRIAL HYPERPLASIA, ENDOMETRIAL INTRAEPITHELIAL NEOPLASIA, AND LOW-GRADE ENDOMETRIOID ADENOCARCINOMA IN PERIMENOPAUSAL WOMEN


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Abstract

Objective. To study the impaired expression of the proteins 0-6-methylguanine-DNA methyltransferase (MGMT) and ezrin in different forms of endometrial hyperplasia (EH), endometrial intraepithelial neoplasia (EIN), and low-grade endometrioid adenocarcinoma (EAC) in perimenopausal women. Subjects and methods. Thirty-five perimenopausal patients, including 12 with simple EH (SHE) without atypia (Group 1), 9 with complex EH (CEH) without atypia (Group 2), 5 with CEH with atypia (CEHA) (Group 3), 5 with EIN (Group 4), and 4 with low-grade EAC (Group 5), which were histologically and immunohistochemically verified using Ki67 and PTEN, were examined. Nine removed uteri and 22 endometrial scrapes were explored. The results of an immunohistochemical examination of ezrin expression and data on the level of MGMT protein expression in endometrial tissues were presented. Results. The level of MGMTprotein expression increases in SHE and CEH, peaks at the stages of CEHA and EIN, and decreases in EAC. The specific features of ezrin expression are associated with that, as the disease progresses from simple HE to EAC, there is a loss of its apical specific localization, as well as free cell cytoplasmic accumulation. Conclusion. The obtained results of clinicoanamnestic, diagnostic, and immunohistochemical studies in the above patient groups can substantiate a differentiated approach to choosing a treatment policy for patients with different forms of EH.

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About the authors

Yevgeniya Altarovna Kogan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University

Email: e_koganevg@oparina4.ru
MD, Professor, head of the 1st Pathology Department Moscow 117997, Ac. Oparina str. 4, Russia; Moscow 119991, Trubetskaya str. 8, Russia

Svetlana Ivanovna Askolskaya

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: askolskayas@mail.ru
MD, leading researcher at the Department of General Surgery Moscow 117997, Ac. Oparina str. 4, Russia

Rauanna Rayimbekovna Sagindykova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: sagindykova.r@mail.ru
postgraduate, 1st Pathology Department Moscow 117997, Ac. Oparina str. 4, Russia

Nafisa Munavarovna Fayzullina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nafisa05@inbox.ru
Senior Researcher, 1st Pathology Department Moscow 117997, Ac. Oparina str. 4, Russia

Alexandra Vjacheslavovna Asaturova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Senior Researcher, 1st Pathology Department Moscow 117997, Ac. Oparina str. 4, Russia

Ara Leonidovich Unanjan

I.M. Sechenov First Moscow State Medical University

Email: unan@yandex.ru
PhD Moscow 119991, Trubetskaya str. 8, Russia

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