TIME COURSE OF CHANGES IN THE PERIPHERAL BLOOD LYMPHOCYTE SUBPOPULATION COMPOSITION OF WOMEN WITH RECURRENT MISCARRIAGE DURING PREGRAVID IMMUNOCYTOTHERAPY


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Abstract

Objective. To study the time course of changes in the percentage and absolute values of peripheral blood lymphocyte subpopulations, including the subpopulations of killer cells with different phenotypes, natural regulatory T cells, and CD200 molecule-expressing cells, in women with recurrent miscarriage of alloimmune genesis in the pregravid preparation using immunocytotherapy (ICT). Subjects and methods. Two weeks after immunization (on days 18-20 of the menstrual cycle), the phenotype of peripheral blood lymphocytes was determined in 52 non-pregnant women with a history of recurrent miscarriage and in 15 fertile women in the control group. Results. There were changes in the count of lymphocytes with killing function and the CD56+, CD3 -CD56,16+, CD56,16+, CD3+CD16+ phenotypes, as well as an increase in the absolute values of lymphocytes with the CD5+CD19+phenotype during two ICT cycles. No changes were found in the levels of the major subpopulations of lymphocytes (CD4+, CD8+, CD19+), CD200 molecule-expressing cells, and regulatory Tcells. Conclusion. Prospects for future research related to the study of the activation state of lymphocyte markers of different subpopulations.

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About the authors

Lyubov V. Krechetova

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Elena Olegovna Stepanova

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: elena2404.07@mail.ru
Junior researcher, Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Marina A. Nikolaeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Valentina V. Vtorushina

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Elena L. Golubeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_golubeva@oparina4.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Nelly A. Khachatryan

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nelly1986@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy Moscow 117997, Ac. Oparina str. 4, Russia

Nana K. Tetruashvili

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_tetruashvili@oparina4.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy Moscow 117997, Ac. Oparina str. 4, Russia

Gennady T. Sukhikh

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: gtsukhikh@mail.ru
MD, PhD, Professor Moscow 117997, Ac. Oparina str. 4, Russia

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