PROINFLAMMATORY GENE EXPRESSION PROFILE OF INNATE IMMUNITY IN THE VAGINAL MUCOSA AS A PREDICTOR OF THE OUTCOME OF AN IN VITRO FERTILIZATION AND EMBRYO TRANSFER PROGRAM
- Authors: Khachaturian J.A.1, Smolnikova V.U.1, Donnikov A.E.1, Nepsha O.S1, Burmenskaya O.V1
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Affiliations:
- Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
- Issue: No 4 (2015)
- Pages: 50-55
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247389
- ID: 247389
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Abstract
Objective. To estimate the clinical signif icance of the expression prof ile of vaginal mucosal cells as a predictor of the outcome of an in vitro fertilization (IVF) program. Subjects and methods. The investigation enrolled 67patients with tuboperitoneal factor infertility. According to the outcomes of the IVF program, the patients were divided into two groups: 1) 30 women in whom biochemical pregnancy (implantation) had occurred; 2) 37 patients with a negative result. Biological material sampling (vaginal mucosal discharge scraping) was carried out on the day of embryo transfer into the uterine cavity. The expression of mRNA of 13 genes, including 6 cytokines (IL-1P, IL-8, IL-10, IL-18, TNF-a, and TGF-P1), 3 transcriptional markers (TBX21, GATA3, and RORC2), 2-toll-like receptors (TLR2, TLR4), and 2 immune system cell surface markers (CD45, CD68), was analyzed. Results. The patients in whom implantation had occurred were statistically significantly less often observed to have the expression profile corresponding to local inflammation (16.7% versus 43.2%). For successful implantation, the odds ratio was 0.26 (0.08-0.82; p = 0.02) in the presence of molecular genetic signs of local inflammation. Conclusion. The local inflammatory response is a poor prognostic criterion for implantation in the IVF program. Investigation of the expression profile of the cytokine genes in the vaginal mucosa is a promising area ofnoninvasive diagnosis and may be used to individualize patient management tactics in the IVF program.
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About the authors
Juliet Artavazovna Khachaturian
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: dzhulietta@inbox.ru
postgraduate, department of assistive technology in the treatment of infertility Moscow 117997, Ac. Oparina str. 4, Russia
Veronika Uruevna Smolnikova
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: veronika.smolnikova@mail.ru
MD, PhD Moscow 117997, Ac. Oparina str. 4, Russia
Andrey Eugenyevich Donnikov
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: donnikov@dna-technology.ru
Ph.D., senior researcher, Laboratory of molecular-genetic methods Moscow 117997, Ac. Oparina str. 4, Russia
O. S Nepsha
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: nepshonok@yandex.ru
PhD, junior researcher of laboratory of molecular genetic methods Moscow 117997, Ac. Oparina str. 4, Russia
O. V Burmenskaya
Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: bourmenska@mail.ru
PhD, researcher of laboratory of molecular genetic methods Moscow 117997, Ac. Oparina str. 4, Russia
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