TIME COURSE OF CHANGES IN THE SUBPOPULATION COMPOSITION OF PERIPHERAL BLOOD LYMPHOCYTES IN THE FIRST TRIMESTER OF PREGNANCY IN WOMEN WITH RECURRENT MISCARRIAGE DURING IMMUNOCYTOTHERAPY


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Abstract

Objective. To study the time course of changes in the subpopulation composition of peripheral blood lymphocytes in the first trimester of pregnancy during immunocytotherapy in women with recurrent miscarriage of alloimmune genesis, including the subpopulations of killer cells with different phenotypes, those of natural regulatory T cells (Tregs) and CD200-carrying cells. Subjects and methods. The phenotype of lymphocytes was determined at 5-6 weeks before and at 8-9 and 12 weeks after immunizing 25 full-term pregnant patients and 11 control women twice with cells from their sexual partners. Results. There were no changes in CD3 +, CD4 +, and CD8 + lymphocytes, as well as killer cells (CD56 +, CD3 -CD56,16 +, CD56,16 +, CD3 +CD56,16 +, CD16 +, CD3 +CD16 +, and CD3 - CD16 +). At 12 weeks, there was an increase in Tregs and CD200-carrying cells to the values in the control group and a decrease in CD5 +CD19 + lymphocytes as compared with the control with the similar total content of B lymphocytes.

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About the authors

Lyubov V. Krechetova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Nana K. Tetruashvili

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_tetruashvili@oparina4.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy Moscow 117997, Ac. Oparina str. 4, Russia

Valentina V. Vtorushina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Elena Olegovna Stepanova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: elena2404.07@mail.ru
Junior researcher, Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Marina A. Nikolaeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Elena L. Golubeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_golubeva@oparina4.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology Moscow 117997, Ac. Oparina str. 4, Russia

Nelly A. Khachatryan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nellyl986@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy Moscow 117997, Ac. Oparina str. 4, Russia

References

  1. Saraswat L., Bhattacharya S., Maheshwari A., Bhattacharya S. Maternal and perinatal outcome in women with threatened miscarriage in the first trimester: a systematic review. Br. J. Obstet. Gynaecol. 2010; 117(3): 245-57.
  2. van Oppenraaij R.H., Jauniaux E., Christiansen O.B., Horcajadas J.A., Farquharson R.G., Exalto N.; ESHRE Special Interest Group for Early Pregnancy (SIGEP). Predicting adverse obstetric outcome after early pregnancy events and complications: a review. Hum. Reprod. Update. 2009; 15(4): 409-21.
  3. Carp Howard J.A., ed. Recurrent pregnancy loss: causes, controversies and treatment. Informa UK ltd.; 2007. 290p.
  4. Chong P.J., Matzner W.L., Ching W.T. Benefit of leukocyte immunizations? Fertil. Steril. 1993; 59(1): 247-9.
  5. Chong P.J., Matzner W.L., Ching W.T. Controversy about immunotherapy. Fertil. Steril. 1993; 59(5): 1138-9.
  6. Farquharson R.G., Stephenson M.D., eds. Early pregnancy. Cambridge University Press; 2010. 308p. Cambridge Books Online http://ebooks.cambridge.org/ Book doi: http://dx.doi.org/10.1017/ CBO9780511777851.
  7. Сидельникова В.М., Сухих Г.Т. Невынашивание беременнос ти. Руководство для практикующих врачей. М.: МИА; 2011. 516с.
  8. Петросян Л.А. Оптимизация лимфоцитоиммунотерапии в лечении привычной потери беременности первого триместра: автореф. дисс.. канд. мед. наук. М.; 2009. 24с.
  9. Патент РФ 2283653. Способ лечения невынашивания беременности. Сидельникова В.М., Сухих Г.Т., Кирющенков П.А., Верясов В.Н.; Заявка № 2004111829; Заявлено 209.04.2004; Опубл. 20.6.2006; Бюл. 26.
  10. Сухих Г.Т., Ванько Л.В. Иммунология беременности. М.: Издательство РАМН; 2003. 400с.
  11. Посисеева Л.В., Малышкина А.И., Бойко Е.Л., Сотникова Н.Ю., Перетятко Л.П., Фетисова И.Н. Реабилитация репродуктивного здоровья супружеских пар с невынашиванием беременности. Иваново: Изд-во Иваново; 2008. 240с.
  12. Khonina N.A., Broitman E.V., Shevela E.Y., Pasman N.M., Chernykh E.R. Mixed lymphocyte reaction blocking factors (MLR-Bf) as potential biomarker for indication and efficacy of paternal lymphocyte immunization in recurrent spontaneous abortion. Arch. Gynecol. Obstet. 2013; 288(4): 933-7.
  13. Nonaka T., Takakuwa K., Ooki I., Akashi M., Yokoo T., Kikuchi A., Tanaka K. Results of immunotherapy for patients with unexplained primary recurrent abortions - prospective non-randomized cohort study. Am. J. Reprod. Immunol. 2007; 58(6): 530-6.
  14. Recurrent Miscarriage Immunotherapy Trialists Group. Worldwide collaborative observational study and meta-analysis on allogenic leukocyte immunotherapy for recurrent spontaneous abortion. Am. J. Reprod. Immunol. 1994; 32(3): 55-72.
  15. Saito S., Shima T., Nakashima A., Shiozaki A., Ito M., Sasaki Y. What is the role of regulatory T cells in the success of implantation and early pregnancy? J. Assist. Reprod. Genet. 2007; 24(9): 379-86.
  16. Clark D.A. Popular myths in reproductive immunology. J. Reprod. Immunol. 2014; 104-105: 54-62.
  17. Vacca P., Cantoni C., Vitale M., Prato C., Canegallo F., Fenoglio D. et al. Crosstalk between decidual NK and CD14+ myelomonocytic cells results in induction of Tregs and immunosuppression. Proc. Natl. Acad. Sci. USA. 2010; 107(26): 11918-23.
  18. Trabanelli S., Ocadlikova D., Evangelisti C., Parisi S., Curti A. Induction of regulatory T cells by dendritic cells through indoleamine 2,3-dioxygenase: a potent mechanism of acquired peripheral tolerance. Curr. Med. Chem. 2011; 18(15): 2234-9.
  19. Clark D.A., Wong K., Banwatt D., Chen Z., Liu J., Lee L. et al. CD200-dependent and nonCD200-dependent pathways of NK cell suppression by human IVIG. J. Assist. Reprod. Genet. 2008; 25(2-3): 67-72.
  20. Clark D.A. Cell-surface CD200 may predict efficacy of paternal mononuclear leukocyte immunotherapy in treatment of human recurrent pregnancy loss. Am. J. Reprod. Immunol. 2009; 61(1): 75-84.
  21. Porter T.F., LaCoursiere Y., Scott J.R. Immunotherapy for recurrent miscarriage. Cochrane Database Syst. Rev. 2006; (2): CD000112
  22. Duckitt K., Qureshi A. Recurrent miscarriage. BMJ Clin. Evid. 2011; 2011: pii: 1409.
  23. Kwak J.Y., Gilman-Sachs A., Moretti M., Beaman K.D., Beer A.E. Natural killer cell cytotoxicity and paternal lymphocyte immunization in woman with recurrent spontaneous abortions. Am. J. Reprod. Immunol. 1998; 40(5): 352-8.
  24. Szpakowski A., Malinowski A., Cieslak J., Nowak M., Wilczyriski J.R., Banasik M. et al. The influence of paternal lymphocyte immunization on the selected subpopulations of peripheral blood lymphocytes in women with recurrent spontaneous abortion of unknown etiology. Ginekol. Pol. 2003; 74(4): 288-96.
  25. Higuchi K., Aoki K., Kimbara T., Hosoi N., Yamamoto T., Okada H. Suppression of natural killer cell activity by monocytes following immunotherapy for recurrent spontaneous aborters. Am. J. Reprod. Immunol. 1995; 33(3): 221-7.
  26. Кречетова Л.В., Хачатрян Н.А., Тетруашвили Н.К., Вторушина В.В., Степанова Е.О., Николаева М.А., Сухих Г.Т. Особенности фенотипа лимфоцитов периферической крови женщин с привычным выкидышем. Акушерство и гинекология. 2014; 10: 27-33.
  27. Кречетова Л.В., Степанова Е.О., Николаева М.А., Вторушина В.В., Голубева Е.Л., Хачатрян Н.А., Тетруашвили Н.К., Сухих Г.Т. Динамика субпопуляционного состава лимфоцитов периферической крови женщин с привычным выкидышем при предгестационной иммуноцитотерапии Акушерство и гинекология. 2015; 4: 37-43.
  28. Krechetova L.V., Nikolaeva M.A., Van’ko L.V., Ziganshina M.M., Golubeva E.L., Stepanova E.O. et al. Optimal detection of serum antipaternal antileukocytic antibodies after injection of allogenic lymphocytes in woman with habitual abortions. Bull. Exp. Biol. Med. 2012; 153(5): 726-9.
  29. Кречетова Л.В., Хачатрян Н.А., Тетруашвили Н.К., Вторушина В.В., Степанова Е.О., Голубева Е.Л., Николаева М.А., Сухих Г.Т. Динамика выработки антиотцовских антилейкоцитарных антител при иммунизации аллогенными клетками женщин с привычным выкидышем Акушерство и гинекология, 2015; 3: 16-20.
  30. Azuma T., Takahashi T., Kunisato A., Kitamura T., Hirai H. Human CD4+ CD25+ regulatory T cells suppress NKT cell functions. Cancer Res. 2003; 63(15): 4516-20.
  31. Mor G., Cardenas I., Abrahams V., Gutter S. Inflammation and pregnancy: the role of the immune system at the implantation site. Ann. N. Y. Acad. Sci. 2011; 1221: 80-7.
  32. Romero R., Gotsch F., Pineles B., Kusanovic J.P. Inflammation in pregnancy: its roles in reproductive physiology, obstetrical complications, and fetal injury. Nutr. Rev. 2007; 65(12, Pt 2): S194-202.

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