PROLIFERATIVE AND MIGRATORY ACTIVITY OF TROPHOBLAST CELLS IN PREECLAMPSIA


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Abstract

Objective. To study JEG-3 trophoblast cell proliferation and migration in the presence of placenta-secreted factors. Subject and methods. Trophoblast cells were cultured in the presence of placental secretory products in the third trimester of physiological or preeclampsia-complicated pregnancy. Cell proliferation and migration were assessed by culture methods and flow cytofluorometry. Results. In the third trimester, the placenta secretes factors that both stimulate and inhibit trophoblast cell proliferation and migration. The effect of trophoblast proliferation and migration inhibitors prevails over that of stimulants. The effect of placenta-secreted factors stimulating cell proliferation and migration is decreased in the placentas of women with preeclampsia. Conclusion. Impaired trophoblast invasion in preeclampsia is associated with a change in the production of placental factors controlling the proliferation and migration of these cells.

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About the authors

K. N Furaeva

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: furaeva_kseniya@mail.ru
research assistant, laboratory of immunology Saint Petersburg 199034, Mendeleevskaya line 3, Russia

O. I Stepanova

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: alzass@mail.ru
PhD, scientific researcher, laboratory of immunology Saint Petersburg 199034, Mendeleevskaya line 3, Russia

O. M Ovchinnikova

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: lathyrus.pratense@gmail.com
research assistant, laboratory of immunology Saint Petersburg 199034, Mendeleevskaya line 3, Russia

L. P Viazmina

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: larisa.viazmina@gmail.com
research assistant, laboratory of immunology Saint Petersburg 199034, Mendeleevskaya line 3, Russia

D. O Bazhenov

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: dmitry-bazhenov@mail.ru
research assistant, laboratory of immunology Saint Petersburg 199034, Mendeleevskaya line 3, Russia

R. V Kapustin

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: kapustin.roman@gmail.com
PhD, physician, D Saint Petersburg 199034, Mendeleevskaya line 3, Russia

N. L Kramareva

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: corbie@hotmail.ru
PhD, head of maternity department Saint Petersburg 199034, Mendeleevskaya line 3, Russia

S. A Selkov

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: selkovsa@mail.ru
Professor, MD, head of laboratory of immunology Saint Petersburg 199034, Mendeleevskaya line 3, Russia

D. I Sokolov

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: falcojugger@yandex.ru
ScD, senior researcher, laboratory of immunology Saint Petersburg 199034, Mendeleevskaya line 3, Russia

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