Aliphatic polyester microparticles obtained with supercritical carbon dioxide as a volume-forming agent for the treatment of stress urinary incontinence

Timur Khaysamudinovich Fatkhudinov; Фатхудинов Тимур Хайсамудинович; Vladimir Karpovich Popov; Попов Владимир Карпович; Irina Vladimirovna Arutyunyan; Арутюнян Ирина Владимировна; Sergey Eduardovitch Bogorodsky; Богородский Сергей Эдуардович; Larisa Ivanovna Krotova; Кротова Лариса Ивановна; Andrey Vitalevich Makarov; Макаров Андрей Витальевич; Andrey Vladimirovich Elchaninov; Ельчанинов Андрей Владимирович; Evgeniya Yurevna Kananykhina; Кананыхина Евгения Юрьевна; Elmira Sharipovna Raimova; Раимова Эльмира Шариповна; Galina Borisovna Bolshakova; Большакова Галина Борисовна; Tatiana Alexandrovna Teterina; Тетерина Татьяна Александровна; Inna Anatolievna Apolikhina; Аполихина Инна Анатольевна; Gennady Tikhonovich Sukhikh; Сухих Геннадий Тихонович

Aliphatic polyester microparticles obtained with supercritical carbon dioxide as a volume-forming agent for the treatment of stress urinary incontinence

Authors: Fatkhudinov T.K.¹^,2, Popov V.K.³, Arutyunyan I.V.¹^,2, Bogorodsky S.E.³, Krotova L.I.³, Makarov A.V.¹^,2, Elchaninov A.V.¹^,2, Kananykhina E.Y.¹^,2, Raimova E.S.⁴, Bolshakova G.B.², Teterina T.A.¹, Apolikhina I.A.⁵^,1, Sukhikh G.T.¹^,5
Affiliations:
1. Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
2. Research Institute of Human Morphology, Russian Academy of Medical Sciences
3. Institute of Laser and Information Technology Problems, Russian Academy of Sciences
4. N.I. Pirogov Russian National Research Medical University
5. I.M. Sechenov First Moscow State Medical University
Issue: No 1 (2015)
Pages: 85-92
Section: Articles
URL: https://journals.eco-vector.com/0300-9092/article/view/247409
ID: 247409

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Objective. To estimate possibilities for using the aliphatic polyester microparticles obtained by supercritical carbon dioxide as a volume-forming agent for the treatment of stress urinary incontinence. Materials and methods. Bioresorbable polymer microparticles were obtained from amorphous D,L-polylactides, polylactoglycolide, and low-molecular weight poly-ε-caprolactone, by using the particles from gas saturated solution (PGSS) and supercritical fluid (SCF) monolithization, followed by cryogrinding. The cytotoxicity and proliferative activity of multipotent stromal cells (MSCs) were assessed directly calculating the cell nuclei and using the xCELLigence monitoring system. Results. Ten types of experimental samples of 100-150-μm microparticles were prepared. Two microcarriers having the least resorption rate and a better resistance to mechanical exposures: polylactide PDL05-SCF and polycaprolactone PCL14-SCF were selected. These samples and their resorption products did not affect the dynamics of MSC expansion and had good adhesive properties. Conclusion. The microparticles obtained by the technology of SCF monolithization, followed by cryogrinding, are characterized by optimal sizes and morphology, stability under culture medium conditions, they fail to have a cytotoxic effect and to suppress the proliferative activity of cells and they also have marked adhesive properties.

Keywords

aliphatic polyesters, supercritical carbon dioxide, multipotent stromal cells, microcarriers, tissue engineering, stress urinary incontinence

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References

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Aliphatic polyester microparticles obtained with supercritical carbon dioxide as a volume-forming agent for the treatment of stress urinary incontinence

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Abstract

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