Plasma and peritoneal fluid IL-1β, IL-8, and substance P levels in patients with different forms of external genital endometriosis and chronic pelvic pain


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Abstract

Objective. To estimate the levels of IL-lß, IL-8, and substance P in the plasma (P) and peritoneal fluid (PF) of reproductive-aged women with pain syndrome varying in intensity in the disseminated form of external genital endometriosis. Subjects and methods. The P and PF levels of IL-lß, IL-8, and substance P were determined in 62 women with external genital endometriosis with varying degrees of the extent and intensity of pain syndrome and in 24 women without endometriosis or pain syndrome. The visual analogue scale was used to evaluate pain syndrome. The concentrations of IL-lß, Il-8, and substance P were estimated by enzyme immunoassay. Results. The P and PF levels of IL-lß, IL-8, and substance P were higher in the women with endometriosis. In the subgroups of patients with varying degrees in the extent of endometriosis, the quantity of cytokines and substance P differed from those in the comparison group with no difference being between the subgroups. In the subgroup of patients with severe pain syndrome, the P and PF levels of cytokines and substance P were higher than those in the comparison group. Conclusion. The found changes are the basis for additional medical and preventive measures to reduce pain syndrome.

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About the authors

Anush S. Ovakimyan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: anush17@bk.ru
Graduate student, Department of Operative Gynecology

Lyubov V. Krechetova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology

Valentina V. Vtorushina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Lyudmila V. Vanko

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: lvanko@mail.ru
Professor, Doctor of Science, Leading researcher, Laboratory of Clinical Immunology

Irena F. Kozachenko

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

MD at the Department of Operative Gynecology

Leila V. Adamyan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: Adamyanleila@gmail.com
Academician of the Russian Academy of Sciences, MD, PhD, Professor, Honored Master of Science of the Russian Federation, Head Specialist in Obstetrics and Gynecology of Ministry of Healthcare and Social Development of Russia, Head of the Department of Surgical Gynecology of Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Head of the Department of Reproductive Medicine and Surgery, Faculty of Postgraduate Education, Moscow State University of Medicine and Dentistry.

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