Interleukin-8 gene polymorphism in women with recurrent pregnancy losses


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To assess the role of interleukin-8 (IL-8) polymorphism in women with recurrent pregnancy losses. Subject and methods. The distribution of the IL-8 alleles and genotypes of the polymorphic loci: -251 A>T (rs 4073) was studied in 100 women. Group 1 consisted of 30 patients with recurrent early losses (at less than 22 weeks’ gestation). Group 2 included 40 patients with multiple very early preterm labors and antenatal fetal deaths (at 22 to 28 weeks’ gestation). Group 3 comprised 30patients with uncomplicated pregnancy and a history of at least 2 full-term births (a control group). Results. There were significant differences in the distribution of the IL-8 genotypes and alleles: -241 A>T (rs 4073) in the group of pregnant women with late reproductive losses versus that of women with physiological pregnancy. Conclusion. Determination of IL-8 gene polymorphisms: -251 A>T (rs 4073) when planning pregnancy will be able to identify a group at risk for late gestation losses for more careful pregravid preparation and optimization of pregnancy management tactics.

Full Text

Restricted Access

About the authors

Evgeniya V. Alegina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: aleginal@rambler.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Nana K. Tetruashvili

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tetrauly@mail.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy

Anna A. Agadzhanova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tetrauly@mail.ru
Doctor of Medicine, leading researcher of the Department of Pregnancy Loss Prevention and Therapy

Dmitrii Y. Trofimov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: d.troflmov@dna-tech.ru
Ph.D. in medical sciences, Head of Laboratory of molecular genetic methods

Andrey E. Donnikov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
Ph.D. in medical sciences, leading researcher of Laboratory of molecular genetic methods

References

  1. Everett C. Incidence and outcome of bleeding before the 20th week of pregnancy: prospective study from general practice. Br. Med. J. 1997; 315(7099): 32-4.
  2. Regan L., Rai R. Epidemiology and the medical causes of miscarriage. Baillieres Best Pract. Res. Clin. Obstet. Gynaecol. 2000; 14(5): 839-54.
  3. Hustin J., Jauniaux E., Schaaps J.P. Histological study of the materno-embryonic interface in spontaneous abortion. Placenta. 1990; 11(6): 477-86.
  4. Hanna J., Goldman-Wohl D., Hamani Y., Avraham I., Greenfield C., Natanson-Yaron S. et al. Decidual NK cells regulate key developmental processes at the human fetal-maternal interface. Nat. Med. 2006; 12(9): 1065-74.
  5. Jovanovic M., Vicovac L. Interleukin-6 stimulates cell migration, invasion and integrin expression in HTR-8/SVneo cell line. Placenta. 2009; 30(4): 320-8.
  6. Champion H., Innes B.A., Robson S.C., Lash G.E., Bulmer J.N. Effects of interleukin-6 on extravillous trophoblast invasion in early human pregnancy. Mol. Hum. Reprod. 2012; 18(8): 391-400.
  7. Vlahopoulos S., Boldogh I., Casola A., Brasier A.R. Nuclear factor-kappaB-dependent induction of interleukin-8 gene expression by tumor necrosis factor alpha: evidence for an antioxidant sensitive activating pathway distinct from nuclear translocation. Blood. 1999; 94(6): 1878-89.
  8. Ahmed O.I., Adel A.M., Diab D.R., Gobran N.S. Prognostic value of serum level of interleukin-6 and interleukin-8 in metastatic breast cancer patients. Egypt. J. Immunol. 2006; 13(2): 61-8.
  9. Caruso D.J., Carmack A.J., Lokeshwar V.B., Duncan R.C., Soloway M.S., Lokeshwar B.L. Osteopontin and interleukin-8 expression is independently associated with prostate cancer recurrence. Clin. Cancer Res. 2008; 14(13): 4111-8.
  10. Kassim S.K., El-Salahy E.M., Fayed S.T., Helal S.A., Helal T., Azzam Eel-D., Khalifa A. Vascular endothelial growth factor and interleukin-8 are associated with poor prognosis in epithelial ovarian cancer patients. Clin. Biochem. 2004; 37(5): 363-9.
  11. Nastase A., Paslaru L., Niculescu A.M., Ionescu M., Dumitra§cu T., Herlea V. et al. Prognostic and predictive potential molecular biomarkers in colon cancer. Chirurgia (Bucur.) 2011; 106(2): 177-85.
  12. De Oliveira L.G., Lash G.E., Murray-Dunning C., Bulmer J.N., Innes B.A., Searle R.F. et al. Role of interleukin 8 in uterine natural killer cell regulation of extravillous trophoblast cell invasion. Placenta. 2010; 31(7): 595-601.
  13. Чистякова Г.Н., Газиева И.А., Ремизова И.И., Черданцева Г.А. Оценка продукции цитокинов при беременности, осложненной угрозой прерывания в первом триместре. Фундаментальные исследования. 2005; 5: 96-8. [Chistyakova G.N., Gazieva I.A., Remizova I.I., Cherdantseva G.A. Assessment of cytokine production in pregnancy complicated by threatened miscarriage in the first trimester. Fundamentalnyie issledovaniya. 2005; 5: 96-8. (in Russian)]
  14. Кравченко Е.Н., Мишутина А.В. Прогнозирование течения беременности и профилактика преждевременных родов посредством определения цитокинов. Медицина и образование в Сибири. 2012; 6: 45-7. [Kravchenko E.N., Mishutina A.V. Predicting the course of pregnancy and prevention of preterm birth by identifying cytokines. Meditsina i obrazovanie v Sibiri. 2012; 6: 45-7. (in Russian)]
  15. Воробйова I.I, Наквасюк Т.С., Толкач С.М., Черненко Т.С., Кучеренко А.М. Особливосл полiморфiзму гену штерлейкшу - 8 (-781C/T) у ваптних жшок. Перинатология и педиатрия. 2013; 2: 22-5.
  16. Кан Н.Е., Ломова Н.А., Донников А.Е., Зубков В.В., Павлович С.В., Дубровина Н.В., Тютюнник В.Л., Сухих Г.Т. Роль генетических факторов в формированиидыхательныхнарушенийуноворожденныхприплацентар-ной недостаточности. Акушерство и гинекология. 2013; 2: 41-5. [Kan N.E., Lomova N.A., Donnikov A.E., Zubkov V.V., Pavlovich S.V., Dubrovina N.V., Tyutyunnik V.L., Sukhikh G.T. Role of genetic factors in the development of neonatal respiratory disorders in placental insufficiency. Akusherstvo i ginekologiya/Obstetrics and gynecology. 2013; 2: 41-5. (in Russian)]
  17. Кофиади И.А., Ребриков Д.В. Методы детекции однонуклеотидных полиморфизмов: аллель-специфичная ПЦР и гибридизация с олигонуклео-тидной пробой. Генетика. 2006; 42(1): 22-32. [Kofiadi I.A., Rebrikov D.V. Methods for detection of SNPs: allele-specific PCR and hybridization with oligonucleotide probe. Genetika. 2006; 42(1): 22-32. (in Russian)]
  18. Lyon E. Mutation detection using fluorescent hybridization probes and melting curve analysis. Expert Rev. Mol. Diagn. 2001; 1(1): 92-101.
  19. Renaud S.J., Macdonald-Goodfellow S.K., Graham C.H. Coordinated regulation of human trophoblast invasiveness by macrophages and interleukin 10. Biol. Reprod. 2007; 76(3): 448-54.
  20. Pitman H., Innes B.A., Robson S.C., Bulmer J.N., Lash G.E. Altered expression of interleukin-6, interleukin-8 and their receptors in decidua of women with sporadic miscarriage. Hum. Reprod. 2013; 28(8): 2075-86.
  21. Madhappan B., Kempuraj D., Christodoulou S., Tsapikidis S., Boucher W., Karagiannis V. et al. High levels of intrauterine corticotropin-releasing hormone, urocortin, tryptase, and interleukin-8 in spontaneous abortions. Endocrinology. 2003;144(6): 2285-90.
  22. ДятловаЛ.И., МихайловА.В., ЧесноковаН.П., Понукалина Е.В., Глухова Т.Н. О роли нарушений баланса цитокинов в патогенезе преждевременного отхождения околоплодных вод, их диагностическое и прогностическое значение. Фундаментальные исследования. 2013; 5-2: 271-5. [Dyatlova L.I., Mihaylov A.V., Chesnokova N.P., Ponukalina E.V., Gluhova T.N. On the role of an imbalance of cytokines in the pathogenesis of premature discharge of amniotic fluid, their diagnostic and prognostic value. Fundamentalnyie issle-dovaniya. 2013; 5-2: 271-5. (in Russian)]
  23. Holst R.M., Mattsby-Baltzer I., Wennerholm U.B., Hagberg H., Jacobsson B. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amni-otic fluid, intra-amniotic inflammation, and preterm delivery. Acta Obstet. Gynecol. Scand. 2005; 84(6): 551-7.
  24. Jacobsson B., Mattsby-Baltzer I., HagbergH. Interleukin-6 and interleukin-8 in cervical and amniotic fluid: relationship to microbial invasion of the chorioam-niotic membranes. BJOG: Br. J. Obstet. Gynaecol. 2005; 112(6): 719-24.
  25. RomeroR., Chaiworapongsa T., GotschF., Yeo L., Madan I., Hassan S. S. The diagnosis and management of preterm labor with intact membranes. In: Winn H.N., Chervenak F.A., Romero R., eds. Clinical maternal-fetal medicine online. 2nd ed. London: Informa Healthcare; 2012: Ch.1.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies