Features of functional state of the mitochondrial apparatus in overweight and obese women in IVF programs
- Autores: Smolnikova V.Y.1, Gorshinova V.K1, Volodina M.A1,2, Tarasova N.V1,2, Vysokikh M.Y.1,2, Kalinina E.A1
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Afiliações:
- Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- A.N. Belozersky Research Institute of Physicochemical Biology, M.V. Lomonosov Moscow State University
- Edição: Nº 9 (2015)
- Páginas: 56-61
- Seção: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247498
- ID: 247498
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Resumo
Objective. To estimate the indicators of mitochondrial function of peripheral mononuclear cells in women with different body indices in an in vitro fertilization (IVF) program and to analyze the relationship between the indicators and the outcomes of treatment. Subject and methods. The investigators analyzed the indicators of mitochondrial energy conversion in peripheral mononuclear cells (MNCs) from 55 patients, stained the isolated MNCs with the fluorescent dye JCL (Life Technologies), and used a flow cytometric method to estimate cell distribution by green and red fluorescence intensity. At the following stage, they comparatively evaluated the efficiency of an IVF and embryo transfer program in the study groups (n=148). Results. In obese women, the duration of superovulation and the total dose of gonadotropins used in their treatment were significantly higher than those in normal weight women (p=0.03; p=0.02) with the similar number of obtained mature oocytes. In overweight patients, the transmembrane potential measured from the maximum oxygen consumption was significantly higher (p=0.02), suggesting the hyperproduction of reactive oxygen species and the presence of oxidative stress. Conclusion. The mitochondrial transmembrane potential measured in the blood of the patients correlated with the rate of clinical pregnancy occurrence and may be of diagnostic value, by significantly predicting the probability of a successful treatment outcome in the IVF program.
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Sobre autores
V. Smolnikova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: veronica.smolnikova@mail.ru
MD, Leading researcher, Department of assistive technologies in infertility treatment
V. Gorshinova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: chiasma@mail.ru
Post-graduate student, Department of assistive technologies in infertility treatment
M. Volodina
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; A.N. Belozersky Research Institute of Physicochemical Biology, M.V. Lomonosov Moscow State University
Email: mariavolodina@yandex.ru
PhD, researcher, Mitochondrial medicine research group
N. Tarasova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; A.N. Belozersky Research Institute of Physicochemical Biology, M.V. Lomonosov Moscow State University
Email: compasstar@gmail.com
PhD, researcher, Mitochondrial medicine research group
M. Vysokikh
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; A.N. Belozersky Research Institute of Physicochemical Biology, M.V. Lomonosov Moscow State University
Email: m_vysokikh@oparina4.ru
PhD, Head of mitochondrial medicine research group, Head of aging molecular mechanisms group
E. Kalinina
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: e_kalinina@oparina4.ru
MD, the Head of Department of assistive technologies in infertility treatment
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