Clinical and immunological rationale for the frequency of immunocytotherapy in married couples with recurrent miscarriage


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Abstract

Objective. To investigate the impact of immunization frequency on the levels of anti-paternal leukocyte antibodies (APLA), as well as the blocking effect of female serum during pregravid preparation of women with recurrent miscarriage (RM). Subjects and methods. APLA and the blocking effect of autologous serum were determined by flow cytometry. The 2007 to 2014 immunological findings were retrospectively analyzed in patients with RM of unknown genesis. A total of 152 patents with RM were examined. Results. Analysis of the chosen indicators of the immune system in women with RM during pregravid preparation using an immunocytotherapy procedure has led to the conclusion that double alloimmunization using the cells of a sexual partner’s leukocyte fraction as a necessary and adequate procedure in married couples with RM of alloimmune genesis in a pregravid preparation program may be recommended. Conclusion. The results allow to recommend a 2-fold immunization with sexual partner cells (TIC) as a necessary and sufficient procedure for married couples with RM of alloimmune genesis in pregravid preparation programs.

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About the authors

Nana K. Tetruashvili

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_tetruashvili@oparina4.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy

Lyubov V. Krechetova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology

Valentina V. Vtorushina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Elena L. Golubeva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_golubeva@oparina4.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Marina M. Ziganshina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: mmz@mail.ru
junior researcher in Laboratory of Clinical Immunology

Elena Olegovna Stepanova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: elena2404.07@mail.ru
Junior researcher, Laboratory of Clinical Immunology

V. A Saribegova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: saribegova_v@rambler.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Marina A. Nikolaeva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology

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