ESTIMATION OF THE DIAGNOSTIC VALUE OF MARKERS FOR THE EARLY DETECTION AND PREDICTION OF THE PROGRESSION OF CERVICAL DYSPLASTIC PROCESSES ON THE BASIS OF AN INVESTIGATION OF THE EXPRESSION OF SOME PROTEINS OF THE OUTER MITOCHONDRIAL MEMBRANE


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Abstract

Objective. To enhance the efficiency of diagnosis and management tactics in patients with cervical intraepithelial neoplasia (CIN), by studying the markers of prediction, such as voltage-dependent anion channel 1 (VDAC1) in the outer external mitochondrial membrane and the protein hexokinase 2 (HK2). Subjects and methods. The investigation enrolled 32 women. According to the results of cytological examination, all the patients were divided into 4 groups: a control group, patients with atypical squamous cells of undetermined significance (ASCUS), low-grade squamous lesion (TSIT), or high-grade squamous lesion (HSIL). In all the patients, VDAC1 and HK2 protein expression levels were determined in the cervical epithelial scrapes using a western-blot analysis and chemiluminescent detection protocol. Results. Analysis of the findings revealed a relationship between VDAC1 protein expression levels, the enzyme HK2, and the degree of CIN. Conclusion. The examined proteins may be potential markers for predicting the progression of CIN and breast cancer at their early stages, which calls for further investigation.

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About the authors

Yulia S. Khlebkova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: y_khlebkova@oparina4.ru
postgraduate Moscow 117997, Ac. Oparina str. 4, Russia

Polina Alexandrovna Vishnyakova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: p_vishnyakova@oparina4.ru
researcher at the Laboratory of mitochondrial medicine Moscow 117997, Ac. Oparina str. 4, Russia

Shaqeh M. Poghosyan

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: shaqpoxos@mail.ru
postgraduate Moscow 117997, Ac. Oparina str. 4, Russia

M. U Vysokikh

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: m_vysokikh@oparina4.ru
PhD, Head of mitochondrial medicine research group Moscow 117997, Ac. Oparina str. 4, Russia

Elena A. Mezhevitinova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_mezhevitinova@oparina4.ru
MD Moscow 117997, Ac. Oparina str. 4, Russia

Vera N. Prilepskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_prilepskaya@oparina4.ru
MD, Professor Moscow 117997, Ac. Oparina str. 4, Russia

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