OXIDATIVE STRESS IN PREECLAMPSIA AND IN NORMAL PREGNANCY


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Objective. To determine the level of reactive oxygen species (ROS) and antioxidant defense in the peripheral blood of women with preeclampsia and physiological pregnancy. Subjects and methods. The investigation enrolled 29 pregnant patients, including 10 with severe or moderate preeclampsia, 11 with physiological pregnancy, and 8 healthy nonpregnant women. The level of reactive oxygen species and antioxidant defense was determined by means of a FORM 3000 device using FORT and FORT kits (Callegari, Italy). Results. The level of ROS and antioxidant defense in physiological pregnancy was found to increase 2.2-fold by the third trimester. In the preeclampsia group, the mean ROS levels were 1.3 times higher than the normal value, but had no statistically significant differences. The groups showed no significant differences in the level of antioxidant defense either. In the preeclampsia group, the level of ROS correlated with the relative urinary concentrations of neutrophils and protein and the level of antioxidant defense negatively correlated with the enzymes ALT and AST. Conclusion. The inverse correlation established between the decreased level of antioxidants and the increased concentrations of the enzymes ALT and ACT points to the role of the former in preventing cells from cytolysis during pregnancy. The relationship found between the elevated ROS levels to the urinary protein concentrations and accordingly the severity of preeclampsia substantiates the possibility of using the determination of oxidative stress in the b1ood of pregnant women to evaluate the severity of preeclampsia.

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作者简介

Aleksey Krasnyi

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences

Email: a_krasnyi@oparina4.ru
PhD, the head of the cytology laboratory Moscow 117997, Ac. Oparina str. 4, Russia

Natalia Kan

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: kan-med@mail.ru
PhD, MD, the head of the obstetric observation department Moscow 117997, Ac. Oparina str. 4, Russia

Victor Tyutyunnik

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tioutiounnik@mail.ru
PhD, MD, the head of the obstetric physiological department Moscow 117997, Ac. Oparina str. 4, Russia

Petimat Khovkhaeva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: p_hovhaeva@oparina4.ru
the postgraduate student Moscow 117997, Ac. Oparina str. 4, Russia

Nadezda Volgina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_volgina@oparina4.ru
scientific researcher of the cytology laboratory Moscow 117997, Ac. Oparina str. 4, Russia

Olga Sergunina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_sergunina@oparina4.ru
obstetrician-gynecologist of the obstetric observation department Moscow 117997, Ac. Oparina str. 4, Russia

Nataliya Tyutyunnik

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tysia07@bk.ru
the resident Moscow 117997, Ac. Oparina str. 4, Russia

Lev Bednyagin

M.V. Lomonosov Moscow State University

Email: levbed@mail.ru
student of the department of Fundamental Medicine Moscow 119991, Lenin Hills 1, Russia

参考

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