REGULATORY TRANSPORT PROTEINS AND CYTOKINES IN THE BLOOD OF PATIENTS WITH PROLIFERATIVE DISEASES OF THE UTERUS


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Abstract

Objective. To determine the involvement of regulatory transport proteins and related proinflammatory cytokines in the pathogenesis of uterine myoma and adenomyosis. Material and methods. The investigators determined the blood levels of α-macroglobulin (α2-MG), α1-antitrypsin (α1-AT), and pregnancy-associated α2-glycoptotein (a2-PAG) by a quantitative rocket immunoelectrophoretic assay and those of lactoferrin (LF), vascular endothelial growth factor (VEGF), interleukin 6 (IL-6), IL-8), and tumor necrosis factor-α (TNF-α) by an enzyme immunoassay in perimenopausal women. Results. All the patients were ascertained to have elevated TNF-a and IL-6 levels; moreover, the concentration of α-AT remained unchanged compared to those observed in healthy women. A manifold (17-18-fold) increase in the content of IL-8 in patients with myoma and comorbidity differed from the changes in the values of this cytokine in those with adenomyosis who showed a not more than 6-fold rise in its concentration. There was an 8-fold elevation in the level of VEGF in adenomyosis and comorbidity, but its concentration in uterine myoma was only doubled. The high level of LF with low α2-MG values were established in uterine myoma, but comorbidity was characterized by higher α2-PAG concentrations. Conclusion. Our findings suggest that an immune response is multi-faceted in patients with uterine myoma and ademyosis and, despite the similarity of clinical symptomatology, which has been discussed by many authors, and uniform triggers, our data demonstrate some dissimilarities in the pathogenetic mechanisms of proliferative diseases of the uterus.

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About the authors

Svetlana Vladimirovna Shramko

Novokuznetsk State Institute for Postgraduate Training of Physicians; Ministry of Health of Russia

Email: shramko_08@mail.ru
PhD (medical science), Teaching assistant of Department of obstetrics and gynecology Novokuznetsk 654005, Stroiteley str. 5, Russia

Veronika Nikolayevna Zorina

Novokuznetsk State Institute for Postgraduate Training of Physicians; Ministry of Health of Russia

Email: macroglobulin@yandex.ru
D.Sc. (Doctor of biological science), Principal Research fellow of Research Laboratory of Immunology Novokuznetsk 654005, Stroiteley str. 5, Russia

Lyudmila Grigoryevna Bazhenova

Novokuznetsk State Institute for Postgraduate Training of Physicians; Ministry of Health of Russia

Email: l_bagenova@mail.ru
M.D. (Doctor of medical science), Professor, Head of Department of obstetrics and gynecology Novokuznetsk 654005, Stroiteley str. 5, Russia

Raisa Michaylovna Zorina

Novokuznetsk State Institute for Postgraduate Training of Physicians; Ministry of Health of Russia

Email: macroglobulin@yandex.ru
D.Sc. (Doctor of biological science), Leading researcher of Research Laboratory of Immunology Novokuznetsk 654005, Stroiteley str. 5, Russia

Tatyana Gennadyevna Ryabicheva

"Vector-best" Closed Corporation

Email: vector@vector.nsc.ru
Leading research fellow of Laboratory of stabilization Novosibirsk 630117, PO box 492, Russia

Elena Viktorovna Chevychalova

City Clinical Hospital One

Email: chev.e.v.@rambler.ru
Head of gynecological department Novokuznetsk 654057, Bardina str. 28, Russia

Nikolay Alexeevich Zorin

Novokuznetsk State Institute for Postgraduate Training of Physicians; Ministry of Health of Russia

Email: macroglobulin@yandex.ru
D.Sc. (Doctor of biological science), Professor, Head of Research Laboratory of Immunology Novokuznetsk 654005, Stroiteley str. 5, Russia

References

  1. Лебедев В.А., Стрижаков А.Н., Давыдов А.И., Пашков В.М. Доброкачественные заболевания матки. М.: ГЭОТАР-Медиа; 2014. 312с.
  2. Chwalisz K., Winkel C.A. Medical managment of women with symptomatic uterine fibroids. In: Segars J.H., ed. Fibroids. Wiley-Blackwell; 2013: 61-75.
  3. Пучков К.В., Подзолкова Н.М., Коренная В.В., Кайибханова К.М. Инновационные подходы в хирургическом лечении миомы матки: сохранить, нельзя удалять. Status Praesens. 2014; 4: 95-102.
  4. Киселев В.И., Сидорова И.С., Унанян А.Л., Муйжнек Е.Л. Гиперпластические процессы органов женской репродуктивной системы: теория и практика. М.: МЕДПРАКТИКА-М; 2010. 467с.
  5. Тихомиров А.Л., Лубнин Д.М. Миома матки. М.: МИА; 2006. 176с.
  6. Uluer E.T., Inan S., Ozbilgin K., Karaca F., Dicle N., Sanci M. The role of hypoxia related angiogenesis in uterine smooth muscle tumors. Biotech. Histochem. 2015; 90(2): 102-10.
  7. Ибрагимова Д.М., Доброхотова Ю.Э. Спорные вопросы патогенеза миомы матки и лечения больных с этим заболеванием. Российский вестник акушера-гинеколога. 2011; 11(2): 37-42.
  8. Mu Y., Hu X., He J., Liu H., Zhang L., Liu H., Hao Z. Serum levels of vascular endothelial growth factor and cancer antigen 125 are related to the prognosis of adenomyosis patients after interventional therapy. Int. J. Clin. Exp. Med. 2015; 8(6): 9549-54.
  9. Di Tommaso S., Massari S., Malvasi A., Bozzetti M.P., Tinelli A. Gene expression analysis reveals an angiogenic profile in uterine leiomyoma pseudocapsule. Mol. Hum. Reprod. 2013; 19(6): 380-7.
  10. Chuang T.D., Khorram O. miR-200c regulates IL8 expression by targeting IKBKB: a potential mediator of inflammation in leiomyoma pathogenesis. PLoS One. 2014; 9(4): e95370.
  11. Ciavattini A., Di Giuseppe J., Stortoni P., Montik N., Giannubilo S.R., Litta P. et al. Uterine fibroids: pathogenesis and interactions with endometrium and endomyometrial junction. Obstet. Gynecol. Int. 2013; 2013: 173184.
  12. Ulukus M., Ulukus E.C., Seval Y., Cinar O., Zheng W., Arici A. Expression of interleukin-8 receptors in patients with adenomyosis. Fertil. Steril. 2006; 85(3): 714-20.
  13. Legrand D., Elass E., Carpenter M., Mazurer J. Lactoferrin: a modulator of immune and inflammatory responses. Cell. Mol. Life Sci. 2005; 62(22): 2549-59.
  14. Korompelis P., Piperi C., Adamopoulos C., Dalagiorgou G., Korkolopoulou P., Sepsa A. et al. Expression of vascular endothelial factor-A, gelatinases (MMP-2, MMP-9) and TIMP-1 in uterine leiomyomas. Clin. Chem. Lab. Med. 2015; 53(9): 1415-24.
  15. Lin C.P., Chen Y.W., Liu W.H., Chou H.C., Chang Y.P., Lin S.T. et al. Proteomic identification of plasma biomarkers in uterine leiomyoma. Mol. Biosyst. 2012; 8(4): 1136-45.
  16. Зорин H.A., Зорина В.H. Сигнальная система макроглобулинов. Обзор литературы. Биомедицинская химия. 2012; 58(4): 400-10.
  17. Адамян Л.B. Особенности процессов пролиферации и апоптоза в эутопическом и эктопическом эндометрии при генитальном эндометриозе. В кн.: Материалы IX Всероссийского научного форума «Мать и дитя». М.; 2007: 314.
  18. Golbidi S., Laher I. Antioxidant therapy in human endocrine disorders. Med. Sei. Monit. 2010. 16(1): RA9-24.
  19. Fletcher N.M., Saed M.G., Abu-Soud H.M., Al-Hendy A., Diamond M.P., Saed G.M. Uterine fibroids are characterized by an impaired antioxidant cellular system: potential role of hypoxia in the pathophysiology of uterine fibroids. J. Assist. Reprod. Genet. 2013; 30(7): 969-74.
  20. Benagiano G., Brosens I. The endometrium in adenomyosis. Womens Health (Lond Engl). 2012; 8(3): 301-12.
  21. Zarkovic K., Larroque-Cardoso P., Pucelle M., Salvayre R., Waeg G., Nègre-Salvayre A., Zarkovic N. Elastin aging and lipid oxidation products in human aorta. Redox Biol. 2015; 4: 109-17.
  22. Koike N., Tsunemi T., Uekuri C., Akasaka J., Ito F., Shigemitsu A., Kobayashi H. Pathogenesis and malignant transformation of adenomyosis (review). Oncol. Rep. 2013; 29(3): 861-7.

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