Transforming growth factor-p expression in the ectocervical epithelial cells in cervical intraepithelial neoplasia and HPV infection


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Abstract

Objective. To investigate cell-cycle regulation in the ectocervical epithelium in infection with HPV and in the development of cervical intraepithelial neoplasia (CIN) by studying the expression of transforming growth factor-beta (TGFbs). Materials and methods. The subject of the investigation was cervical biopsy specimens from 105 women, including 39, 24, and 12 patients with CIN I, II, and III, respectively (a study group); A comparison group included 30 HPV-infectedpatients without CIN. Immunohistochemical examinations were made in accordance with standard protocols using Diagnostic BioSystems reagents (USA). Results. The expression of TGFbs was found in the ectocervical basal cells of patients in the comparison group and in the cells of basal, parabasal, and intermediate layers in the CIN I group, in the basal and parabasal layers in the CIN II group; TGFbs expression was absent in the majority of patients in the CIN III group. Quantification of TGFBs expression established that the latter in the ectocervical basal, parabasal, and intermediate layers was statistically significantly more pronounced in the HPV infection minus CIN group and the CIN I and CIN II groups than in the CIN III group. Conclusion. As CIN progresses, the expression of TGFbs in the ectocervical epithelium at first increases and then decreases to the point of complete disappearance, which apparently suggest that the antiproliferative activity of the epithelium reduces by autocrine regulation.

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About the authors

Yevgeny I. Samodelkin

Academician E.A. Wagner Perm State Medical University, Ministry of Health of Russia

Email: sei-p@mail.ru
MD, Professor of Pathological Physiology

Polina V. Kosareva

Academician E.A. Wagner Perm State Medical University, Ministry of Health of Russia

Email: perm-bagira@narod.ru
MD, main researcher of Department of morphological and pathophysiological studies CSRL, Head of the course of clinical laboratory diagnostics, an assistant professor of microbiology, virology with the course of clinical laboratory diagnostics

Valentina V. Vetelina

Academician E.A. Wagner Perm State Medical University, Ministry of Health of Russia

post-graduate student of the Department of Pathological Physiology

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