HAS2 gene expression in cumulus cells as a predictor of the outcome of in vitro fertilization programs


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Abstract

Objective. To search for molecular genetic markers to assess the quality of embryos with a high potential for implantation in order to enhance the efficiency of infertility treatment using assisted reproductive technologies. Subjects and methods. 39 patients who had undergone an in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) program were examined during a retrospective case-control study and divided into 2 groups according to the outcome of IVF/ICSI treatment: 1) 14 women with clinical pregnancy that had occurred due to this treatment; 2) 25 women without clinical pregnancy. A real-time RT-PCR assay was used to examine the cumulus cell expression level of 5 genes: hyaluronan synthase 2 (HAS2), prostaglandin synthase 2 (PTFS2), gremlin 1 (GREM1), versican (VCAN), and inositol-triphosphate 3-kinase A (ITPKA). Results. In the clinical pregnancy group, HAS2 mRNA expression was observed to increase 1.5-fold (p = 0.018). At the same time there were no statistically significant differences between the quality of transferred embryos and the rate of clinical pregnancy. Conclusion. The estimation of HAS2 expression in the cumulus cells may serve as a minimally invasive test used in clinical practice to enhance the efficiency of IVF programs as a whole.

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About the authors

Natalia Aleksandrovna Safronova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: safrochik900@bk.ru
M.D., postgraduate of the department of assisted reproductive technology in infertility treatment

Elena Anatolievna Kalinina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
M.D, Ph.D, The Head of the department of assisted reproductive technology in infertility treatment

Andrew Evgenievich Donnikov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, Senior Researcher of molecular-genetical laboratory

Olga Vladimirovna Burmenskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_bourmenskaya@oparina4.ru
Ph.D., Researcher of molecular-genetical laboratory

Natalia Petrovna Makarova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: np.makarova@gmail.com
PhD, Researcher of the department of assisted reproductive technology in infertility treatment

Anastasia Valerievna Zobova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: zoana2011@gmail.com
PhD, Researcher of the department of assisted reproductive technology in infertility treatment

Kamila Ullubievna Alieva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k_alieva@oparina4.ru
PhD, Researcher of the department of assisted reproductive technology in infertility treatment

Victoria Konstantinovna Gorshinova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: chiasma@mail.ru
M.D., postgraduate of the department of assisted reproductive technology in infertility treatment

Dmitrii Yurievich Trofimov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: d_trofimov@oparina4.ru
Ph.D., The Head of molecular-genetical laboratory

Gennady Tikhonovich Sukhikh

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
MD, PhD, Director

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