IMPACT OF SURROUNDING TISSUE ON MICRORNA EXPRESSION IN THE EUTOPIC ENDOMETRIUM IN SEVERE ENDOMETRIOSIS


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Abstract

Objective. To comparatively investigate the expression of microRNAs (miR-9-5p, miR-20b-5p, miR-139-3p, miR-193a-3p, miR-200a-5p, miR-210-5p, miR-376c-5p, and miR-378а-5р) in the eutopic and ectopic endometrium and in the vicinity of the affected area of the tissue in patients with severe infiltrating endometriosis. Subjects and methods. The investigation enrolled 22 women with severe retrocervical endometriosis involving the colon. An ectopic endometrial tissue fragment and an affected organ tissue segment alongside ectopic endometriosis were intraoperatively taken in all the women. Ectopic endometrial scrapings were taken in 12 of these women. The tissues were sampled in the reproductive phase of the menstrual cycle. The expression level of microRNAs was estimated by quantitative real-time PCR using a TaqMan miRNA Assay reagent kit (Applied Biosystems). Results. The expression of miR-376c-5p in the ectopic endometrium was shown to be higher than that in the eutopic one in severe external endometriosis. The expression of this mirRNA was ten times higher than that in the eutopic endometrium and twice higher than in the adjacent normal tissue area. The high expression level of other miRNAs in the tissue surrounding the affected area did not allow us to assess a change in their expression in ectopic endometrial tissue. Conclusion. There was an association of an increased miR-376c-5p expression level in the ectopic endometrium in severe external endometriosis. The expression profile of the surrounding tissue can substantially both mask and modify the endometrial tissue microRNA profile.

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About the authors

Leonid Zakievich Faizullin

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: l_faizullin@oparina4.ru
MD, Molecular-genetic Laboratory Researcher Moscow 117997, Ac. Oparina str. 4, Russia

Vitaliy Nikolaevich Karnauhov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_karnauhov@oparina4.ru
junior researcher of Molecular-genetic Laboratory Moscow 117997, Ac. Oparina str. 4, Russia

Leila V. Adamyan

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Moscow State University of Medicine and Dentistry

Email: Adamyanleila@gmail.com
Academician of the Russian Academy of Sciences, MD, PhD, Professor, Honored Master of Science of the Russian Federation, Head Specialist in Obstetrics and Gynecology of Ministry of Healthcare and Social Development of Russia, Head of the Department of Surgical Gynecology; Head of the Department of Reproductive Medicine and Surgery, Faculty of Postgraduate Education Moscow 117997, Ac. Oparina str. 4, Russia; Moscow 127473, Delegatskaya str. 20, Russia

Olesya Nikolaevna Gorshkova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_gorshkova@oparina4.ru
Postgraduate student, Department of General Surgery Moscow 117997, Ac. Oparina str. 4, Russia

Elena Grigorevna Khilkevich

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University

Email: e_khilkevich@oparina4.ru
leading researcher of General Surgery; professor of Department of Obstetrics, Gynecology, Perinatology and Reproductive Moscow 117997, Ac. Oparina str. 4, Russia; Moscow 119991, Bolshaya Pirogovskaya str. 2/4, Russia

Vladimir Dmitrievich Chuprynin

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_chuprynin@oparina4.ru
PhD, Head of the Department of General Surgery Moscow 117997, Ac. Oparina str. 4, Russia

Dmitriy Yur’evich Trofimov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: d_troflmov@oparina4.ru
MD, PhD, Molecular-genetic Laboratory Chief Moscow 117997, Ac. Oparina str. 4, Russia

A. S Arakelyan

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_arakelyan@oparina4.ru
PhD, obstetrician-gynecologist, gynecological department Moscow 117997, Ac. Oparina str. 4, Russia

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