MODERN CAPABILITIES OF MOLECULAR GENETIC METHODS IN THE DIAGNOSIS OF EARLY NEONATAL SEPSIS IN PRETERM NEONATES


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Abstract

Objective. To search for early diagnostic biomarkers for early neonatal sepsis (ENS) in preterm neonates, by using molecular genetic methods. Subject and methods. Buccal scrape (BS) cells and venous blood (VB) were investigated in 71 preterm infants treated in the Intensive Care Unit (ICU), Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, in the period August 2012 to March 2015. Results. The investigation showed that the course of ENS in the preterm infants was accompanied by the general state of immunosuppression. There were changes in the expression of the IL12A gene in the BS cells and in that of the TNF-α and GATA3genes in VC. Conclusion. Current molecular genetic methods were used to identify diagnostic biomarker candidates for ENS in preterm infants. A mathematical model was created to diagnose a neural network based on the determination of the expression level of the IL12A and CD68 genes in the BS cells of preterm infants (sensitivity 81%, specificity 74%). The procedure proposed for use is non-invasive and able to accelerate a diagnostic search and to optimize the timing of pathogenetic therapy for ENS in premature infants in ICU.

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About the authors

Irina V. Nikitina

Email: i_nikitina@oparina4.ru
Ph.D., doctor of the intensive care department of neonatology and pediatrics, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia 117997, Russia, Moscow, Ac. Oparina str. 4

Oksana S. Nepsha

Email: nepshonok@yandex.ru
Ph.D., research scientist of the Laboratory of Molecular-genetic methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Andrey E. Donnikov

Email: a_donnikov@oparina4.ru
Ph.D., Senior Researcher of the Laboratory Of Molecular-genetic Methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Dmitrii Yurievich Trophimov

Email: d_troflmov@oparina4.ru
Ph.D., The Head of molecular-genetical laboratory, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Olga Vladimirovna Milaya

Email: o_milaya@oparina4.ru
graduate student, Resuscitation and Neonatal Intensive Care, Department of Neonatology and Pediatrics, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Anna V. Degtyareva

Email: a_degtyareva@oparina4.ru
MD, Head of the Scientific Advisory Pediatrics Division of Neonatology and Pediatrics, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia 117997, Russia, Moscow, Ac. Oparina str. 4

Oleg V. Ionov

Email: o_ionov@oparina4.ru
Ph.D., Head of the intensive care department of neonatology and pediatrics, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia 117997, Russia, Moscow, Ac. Oparina str. 4

Victor V. Zubkov

Email: victor.zubkov@mail.ru
MD, PhD, Head of department of neonatology and pediatrics, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Dmitriy N. Degtyarev

Email: d_degtiarev@oparina4.ru
MD, PhD, Deputy Director on scientific work, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

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