Association of vitamin D receptor (VDR) gene polymorphism with embryological characteristics and effectiveness of in vitro fertilization programs


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Abstract

Objective. To study the significance of vitamin D receptor (VDR) gene polymorphism as a molecular genetic marker for estimating the rate of implantation and clinical pregnancy and the patterns of oogenesis and embryogenesis in assisted reproductive technology programs. Subjects and methods. A total of 142 patients who had undergone an in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) program with superovulation and embryo transfer in this cycle were examined during a prospective study case-control study. Serum 25-hydroxyvitamin D levels and VDR gene polymorphism were ascertained in all the patients. The women were divided into 3 groups according to the level of vitamin D; 1) blood vitamin D deficiency (<10 ng/ml); 2) blood vitamin D insufficiency (10-29 ng/ml); 3) normal blood vitamin D levels (30-100 ng/ml). Results. Analysis of the association of VDR gene polymorphism with serum vitamin D levels established that the patients with A/G and A/A genotypes of VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) [rs 10735810] had serum vitamin D deficiency in 13.4% of women, vitamin D insufficiency in 70.1%, and normal vitamin D levels in 16.5% of the women included in the study. At the same time, the patients with G/G genotype of VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) were found to have blood vitamin D deficiency in 40% and insufficiency of this vitamin in 60%. The findings suggest that the carriage of G/G genotype of this polymorphism more than doubles the risk of serum vitamin D deficiency. The analysis of the association of VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) with the quality of the embryos obtained from women with different serum vitamin D levels revealed that the carriage of G/G genotype of the polymorphism (compared to A/A and A/G genotypes) reduced the risk of having poor-quality embryos by more than 3 times. Conclusion. VDR gene polymorphism: 2A>G (Lys2Arg) (FokI) [rs 10735810] is an independent factor that can predict the serum vitamin D level that affects the outcomes of assisted reproductive technology programs and embryo quality and can complement the arsenal of already existing markers.

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About the authors

Zokhra Mokhamad Naimi

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: naimi-zohra@mail.ru
Sami, graduate student of department of assisted reproductive technology in treating sterility

Elena Anatolievna Kalinina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
MD, The chief of department of assisted reproductive technology in treating sterility

Andrew Evgenievich Donnikov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, Senior Researcher of molecul-genetical laboratory

Alina Khasanovna Dudarova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: kanshaov85@mail.ru
graduate student of department of assisted reproductive technology in treating sterility

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