Mitochondrial proteins of peripheral plasma microvesicles as biomarkers of early pregnancy losses
- Authors: Bulatova Y.S.1, Tetruashvili N.K.1, Vishnyakova P.A1, Vysokikh M.Y.1, Marei M.V1, Pyataeva S.V1, Sukhikh G.T.1
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Affiliations:
- Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Issue: No 7 (2017)
- Pages: 46-51
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247949
- DOI: https://doi.org/10.18565/aig.2017.7.46-51
- ID: 247949
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Abstract
The paper presents the results of a pilot study on the determination of proinflammatory factors of mitochondrial damage-associated molecular patterns (mtDAMP) in pregnant women with physiological pregnancy and recurrent early miscarriages. Objective. To determine the levels of proinflammatory factors of mtDAMP in the peripheral blood of patients with threatened and recurrent miscarriage versus the similar indicators in women with physiological pregnancy. Subjects and methods. The investigation enrolled 24 pregnant women whose peripheral plasma samples were selected retrospectively after successful achievement of 22 weeks’ gestation in 12patients (a control group) and in 12 patients with recurrent early miscarriage (a study group). To determine the level of mtDAMP proteins in the patients of the examined groups, their venous blood was fractionated, microvesicles were isolated from the obtained plasma, and mitochondrial protein levels were analyzed by Western-blotting. The findings were statistically processed using the SPSS program according to the ANOVA method. Results. Comparative analysis of the relative content of mitochondrial proteins in the plasma microvesicles showed that in the women with threatened and recurrent miscarriage, whose pregnancy spontaneously aborted, the levels of all marker proteins were significantly lower than those in the women of the control group (p < 0.01). Conclusion. The low levels of proinflammatory factors of peripheral plasma mtDAMP are shown to be an unfavorable factor for early pregnancy.
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About the authors
Yulia S. Bulatova
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: yu.bulatova@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy
Nana K. Tetruashvili
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: tetrauly@mail.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy
P. A Vishnyakova
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: p_vishnyakova@oparina4.ru
researcher at the Laboratory of mitochondrial medicine
Mikhail Yu. Vysokikh
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: m_vysokikh@oparina4.ru
PhD, the head of mitochondrial medicine research group
M. V Marei
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: m_marey@oparina4.ru
researcher at the Laboratory of mitochondrial medicine
S. V Pyataeva
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: biosonya@gmail.com
PhD. researcher at mitochondrial medicine research group
Gennadiy Tikhonovich Sukhikh
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of RussiaAcademician of RAS, MD, PhD, Professor, Director
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