A liquid-based cytology technique for the diagnosis of precancerous fallopian tube lesions in ovarian tumors


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To examine cytological smears of a fimbrial section of the fallopian tube to identify the cytological characteristics of ovarian serous tumors and the possibilities of using the technique for the diagnosis of ovarian serous cancer in its early stages. Subjects and methods. The investigation enrolled 21 patients (mean age 45.6±13.7 years), in whom 36smears were taken from 36 fallopian tubes. The material was placed in a container having a patented concentrated solution that was a buffered medium with low methanol content and microscopically evaluated after using Pappanicolaou staining. When atypical glandular cells were detected, an immunocytochemical study was conducted using antibodies to p16ŒK4a (clone E6H4, RTU) and Ki67 (clone 274-11AC3, RTU) as a set of CINtec PLUS (Roche) and antibodies top53 (clone DO-7, RTU, Dako). Results. Histological diagnosis revealed papillary fallopian tube hyperplasia in the presence of serous borderline tumors in all cases; however, there were no clear cytological signs of this disease. The cytological changes in high-grade ovarian carcinomas, which were largely concerned with the features of the cell nuclear apparatus, were most characteristic and immunocytochemically confirmed using the markers P16, p53, and Ki-67. Conclusion. Further study of the cytological features of smears in intraepithelial pathology of the fallopian tube is necessary for the elaboration of cytological criteria that will be able to diagnose early-stage intraepithelial lesions and to create a platform for the development of new approaches to searching for the early diagnostic markers of ovarian serous carcinomas.

Full Text

Restricted Access

About the authors

Alexandra Vyacheslavovna Asaturova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_asaturova@oparina4.ru
Ph.D., senior researcher, Department of Pathological Anatomy

Maya Viktorovna Sannikova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: m_sannikova@oparina4.ru,mvs6103@gmail.com
Ph.D

Grigory Nikolaevich Khabas

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_khabas@oparina4.ru
Ph.D., head of the department of combined and complex methods of treatment of gynecological diseases

Anzhelika Vladimirovna Timofeeva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_timofeeva@oparina4.ru
Ph.D., Senior Researcher, Laboratory of Molecular Pathophysiology

References

  1. Siegel R., Ma J., Zou Z., Jemal A. Cancer statistics. 2014. CA Cancer J. Clin. 2014; 64(1): 9-29.
  2. Чиссов В.И., Старинский В.В., Петрова Г.В. ред. Злокачественные заболевания в России в 2011году (заболеваемость и смертность). М.: ФГБУ «МНИОИ им. П.А. Герцена» Минздрава России; 2013. 289с. [Chissov V.I., Starinsky V.V., Petrova G.V., ed. Malignant diseases in Russia in 2011 (morbidity and mortality). Moscow: FGBU P.A. Herzen MNIOI, Ministry of Health of Russia; 2013. 289p. (in Russian)]
  3. Стерджен К.М., Даффи М.Дж., Стенман У.-Х., Лилья Г., Брюннер Н., Дэниэл У. Чан Д.У. и др. Клиническое руководство Национальной академии клинической биохимии США по применению маркеров опухолей яичка, предстательной железы, прямой и ободочной кишки, молочной железы и яичников в медицинских лабораториях. Лабораторная диагностика. 2013; 3: 3-13. [Stufen K.M., Duffy M.J., Stenman U.-H., Lilya G., Brunner N., Daniel U., Chan D.U. et al. Clinical guidelines of the National Academy of Clinical Biochemistry of the United States on the use of markers of tumors of the testicle, prostate, rectum and colon, breast and ovaries in medical laboratories. Laboratornaya diagnostika. 2013; 3: 3-13. (in Russian)]
  4. Hoskins P., Gotlieb W. Ovarian cancer screening: UKCTOCS trial. Lancet. 2016; 387(10038): 2602-3. doi: 10.1016/S0140-6736(16)30848-0.
  5. Wei S.U., Li H., Zhang B. The diagnostic value of serum HE4 and CA-125 and ROMA index in ovarian cancer. Biomed. Rep. 2016; 5(1): 41-4.
  6. Kurman R.J., Shih I.M. The origin and pathogenesis of epithelial ovarian cancer: a proposed unifying theory. Am. J. Surg. Pathol. 2010; 34(3): 433-43. doi: 10.1097/PAS.0b013e3181cf3d79.
  7. Gershenson D.M. Low-grade serous carcinoma of the ovary or peritoneum. Ann. Oncol. 2016; 27(Suppl. 1): i45-i49. doi: 10.1093/annonc/mdw085.
  8. Karst A.M., Drapkin R. Ovarian cancer pathogenesis: a model in evolution. J. Oncol. 2010; 2010: 932371. doi: 10.1155/2010/932371.
  9. Powell C.B., Kenley E., Chen L.M., Crawford B., McLennan J., Zaloudek C.J. et al. Risk-reducing salpingo-oophorectomy in BRCA mutation carriers: role of serial sectioning in the detection of occult malignancy. J. Clin. Oncol. 2005; 23(1): 127-32.
  10. Finch A., Shaw P., Rosen B., Murphy J., Narod S.A., Colgan T.J. Clinical and pathologic findings of prophylactic salpingo-oophorectomies in 159 BRCA1 and BRCA2 carriers. Gynecol. Oncol. 2006; 100(1): 58-64.
  11. Ren Z., Zhang Y., Yang H., Zhu L., Maeda D., Takazawa Y. Liquid-based cytology aids in primary fallopian tube cancer diagnosis. J. Zhejiang Univ. Sci B. 2009; 10(8): 635-8.
  12. Maeda D., Takazawa Y., Ota S., Takeuchi Y., Seta A., Nakagawa S. et al. Bilateral microscopic adenocarcinoma of the fallopian tubes detected by an endometrial cytologic smear. Int. J Gynecol. Pathol. 2010; 29(3): 273-7.
  13. Minato H., Shimizu M., Hirokawa M., Fujiwara K., Kohno I., Manabe T. Adenocarcinoma in situ of the fallopian tube. A case report. Acta Cytol. 1998; 42(6): 1455-7.
  14. Dudkiewicz J. Quantitative and qualitative changes of epithelial cells of fallopian tubes in women according to the phase of menstrual cycle. A cytologic study. Acta Cytol. 1970; 14(8): 531-7.
  15. Dhanani M., Nassar A., Dinh T. Classification of fallopian tube cytology sampling to develop a model for future peritoneal and ovarian cancer screening. J. Am. Soc. Cytopathol. 2014; 3(5): S35-6. doi: 10.1016/j.jasc.2014.09.076.
  16. Lum D., Guido R., Rodriguez E., Lee T., Mansuria S., D'Ambrosio L., Austin R.M. Brush cytology of the fallopian tube and implications in ovarian cancer screening. J. Minim. Invasive Gynecol. 2014; 21(5): 851-6. doi: 10.1016/j. jmig.2014.03.017.
  17. Herzberg A.J., Raso D.S., Silverman J.F. Color atlas of normal cytology. Churchill Livingstone; 1999.
  18. Haeusler G., Tempfer C., Lehner R., Sam C., Kainz C. Fallopian tissue sampling with a cytobrush during hysteroscopy: a new approach for detecting tubal infection. Fertil. Steril. 1997; 67(3): 580-2.
  19. Matsushima T., Kaseki H., Ishihara K., Araki T. J Assessment of fallopian tube cytology for the diagnosis of endometriosis and hydrosalpinx. Nippon Med. Sch. 2002; 69(5): 445-50.
  20. Mulvany N.J., Arnstein M., Ostör A.G. Fallopian tube cytology: a histocorrelative study of 150 washings. Diagn. Cytopathol. 1997; 16(6): 483-8.
  21. Kurman R.J., Shih Ie.M. Molecular pathogenesis and extraovarian origin of epithelial ovarian cancer--shifting the paradigm. Hum. Pathol. 2011; 42(7): 918-31. doi: 10.1016/j.humpath.2011.03.003.
  22. Асатурова А.В., Коган Е.А., Ежова Л.С., Жарков Н.В., Файзуллина Н.М., Хабас Г.Н. Папиллярная гиперплазия маточной трубы: сочетание с серозными пограничными опухолями яичника и молекулярно-биологическая характеристика. Акушерство и гинекология. 2015; 11: 58-64. [Asaturova A.V., Kogan E.A., Ezhova L.S., Zharkov N.V., Faizullina N.M., Khabas G.N. Papillary tubal hyperplasia: Concurrence with serous borderline ovarian tumors and molecular biological characteristics. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2015; 11: 58-64. (in Russian)]
  23. Kuhn E., Kurman R.J., Soslow R.A., Han G., Sehdev A.S., Morin P.J. et al. The diagnostic and biological implications of laminin expression in serous tubal intraepithelial carcinoma. Am. J. Surg. Pathol. 2012; 36(12): 1826-34. doi: 10.1097/PAS.0b013e31825ec07a.
  24. Matjusaitis M., Chin G., Sarnoski E.A., Stolzing A. Biomarkers to identify and isolate senescent cells. Ageing Res. Rev. 2016; 29: 1-12.
  25. Vang R., Visvanathan K., Gross A., Maambo E., Gupta M., Kuhn E. et al. Validation of an algorithm for the diagnosis of serous tubal intraepithelial carcinoma. Int. J. Gynecol. Pathol. 2012; 31(3): 243-53. doi: 10.1097/PGP.0b013e31823b8831.
  26. Mahe E., Tang S., Deb P., Sur M., Lytwyn A., Daya D. Do deeper sections increase the frequency of detection of serous tubal intraepithelial carcinoma (STIC) in the „sectioning and extensively examining the fimbriated end” (SEE-FIM) protocol? Int. J. Gynecol. Pathol. 2013; 32(4): 353-7. doi: 10.1097/ PGP.0b013e318264ae09.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies