Results of preimplatation genetic screening of embryos in married couples with sperm DNA fragmentation


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Abstract

Objective. To determine the frequency of aneuploidies (monosomies, trisomies, disomies, nullisomies) from all chromosomes, as well as DNA deletions and amplifications in the embryos from couples with higher and normal sperm nuclear DNA fragmentation levels. Subjects and methods. An array-based preimplatation genetic screening program was applied to 170 couples; each couple underwent estimation of sperm concentration, motility and morphology according to the 2010 WHO standards, as well as sperm nuclear DNA fragmentation levels by the TUNEL method. Results. A relationship was found between the level of paternal sperm DNA fragmentation and the frequency of obtaining embryos with a normal karyotype. With the increased level of DNA fragmentation, this figure is 47.96% vs 66.17% with its normal level (p < 0.02). There was also a substantially increased frequency of embryo DNA deletions and amplifications with a higher level of paternal sperm DNA fragmentation (35.89% vs 5.39% with its normal level; p < 0.001). Fragmentation does not have a statistically significant impact on abnormal chromosome number. Conclusion. This study has demonstrated a correlation between the level of paternal sperm DNA fragmentation and the frequency of structural karyotype abnormalities in embryos.

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About the authors

Yulia Yu. Kiseleva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Peoples' Friendship University of Russia

Email: 79164171311@yandex.ru
embryologist; graduate student of the Department of Biology and General Genetics Medical Faculty

Madina M. Azova

Peoples’ Friendship University

Email: azovam@mail.ru
DSc, Associate Professor, Head of the Department of Biology and General Genetics Medical Faculty

T. A Kodyleva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: t_kodyleva@oparina4.ru
embryologist 1st gynecology department

I. V Ushakova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: ir_ushakova@oparina4.ru
Ph.D., embryologist, department of reproductive

Anastasiya O. Kirillova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_kozyreva@oparina.ru
embryologist

Alexey N. Ekimov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_ekimov@oparina4.ru
researcher

Aleksandr S. Rakitko

M.V. Lomonosov Moscow State University

Email: rakitko@gmail.com
PhD student, Department of Theory of Probability, Faculty of Mathematics and Mechanics

Tatiana O. Volodyaeva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tvolo90@mail.ru
embryologist

Nona G. Mishieva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nondoc555@mail.ru
MD, Senior researcher of 1st gynecology department

Aydar N. Abubakirov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nondoc555@yahoo.com
PhD, Head of 1-st gynecology department

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