Inflammatory markers in the normal and thin endometrium in chronic endometritis


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Abstract

Objective. To investigate the clinical and morphological characteristics of chronic endometritis (CE) in patients with the normal and thin endometrium in the dynamics of rehabilitation, by applying peloids. Subjects and methods. The case-control study and the prospective cohort study were conducted in 240 patients aged 27.0±2.9 years with CE, including 118 cases with the normal endometrium (M-echo > 6 mm in the proliferative phase of the cycle) and 122 women with the thin endometrium (M-echo ≤ 6 mm), who were treated with vitamin therapy and physiotherapy (Scheme 1) and in combination with peloids (Scheme 2) from Lake Karachi (West Siberian Plain). Before and after treatment, the investigators conducted a clinical examination, endometrial ultrasonography, by assessing changes in the scores derived from the scale designed by M.N. Bulanov, a cultural study of cervical canal discharge and endometrial pipelle biopsy with an immunohistochemical (IHC) examination of the expression of CD138 plasma cells and CD20 B lymphocytes in 88 cases, including 24 biopsies that were analyzed using nonparametric statistics and multivariate analysis of variance. Results. The data of the clinic, the scoring ultrasound characteristics of the endometrium, and the magnitude of inflammatory and cellular components in the uterine mucosal biopsy specimens in patients with CE and the normal endometrium after treatment revealed significant values in normalizing the menstrual cycle and improving fertility, as well as a decrease in the content of CD20- and CD138-positive cells. During rehabilitation in combination with peloids, the thin endometrium was characterized by the higher expression of inflammatory markers in the pipelle biopsy specimens along with positive clinical changes and growth of symbiotic microflora. Conclusion. The use of peloids in the treatment of CE in women with the thin endometrium induces regeneration with the transient manifestation of IHC markers of inflammation.

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About the authors

Igor’ Olegovich Marinkin

Novosibirsk State Medical University, Ministry of Health of Russia

Email: rector@ngmu.ru
doctor of medical sciences, professor, the head of the Obstetrics and Gynecology Department, rector

Natalia Victorovna Trunchenko

Novosibirsk State Medical University

Email: trunchenko@yandex.ru
assistant of the Obstetrics and Gynecology Department

Alexandr Victorovich Volchek

Novosibirsk City Clinical Perinatal Center

Email: alexvolchek@yandex.ru
physician

Tatyana Avgustovna Ageeva

Novosibirsk State Medical University

Email: ageta@mail.ru
doctor of medical sciences, professor of the Pathoanatomic Department

Evgeniy Viktorovich Nikitenko

Novosibirsk City Clinical Perinatal Center

Email: evniki@yandex.ru
candidate of medical sciences, head of the Pathoanatomic Department

Konstantin Yuryevich Makarov

Novosibirsk State Medical University

Email: fdpngma@mail.ru
doctor of medical sciences, assistant professor of the Obstetrics and Gynecology Department

Vitaliy Mihaylovich Kuleshov

Novosibirsk State Medical University

Email: kuleshov_vm@mail.ru
doctor of medical sciences, professor of the Obstetrics and Gynecology Department

Vladimir Vilor’evich Omigov

Novosibirsk State Medical University

Email: omigov@vector.nsc.ru
candidate of medical sciences, senior researcher of laboratory of cellular biology and fundamental basis of reproduction

Svetlana Vladimirovna Aidagulova

Novosibirsk State Medical University

Email: s.aydagulova@gmail.com
doctor of biological sciences, professor, head of laboratory of cellular biology and fundamental basis of reproduction, Central scientific laboratory

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