A change in the concentration of extracellular DNA during pregnancy


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Abstract

Objective. To determine the plasma concentrations of total extracellular DNA (tDNA) and its fractions during pregnancy, by taking into account the possible impact of the body mass index (BMI) of women, anemia in pregnant women, fetal and placenta weight. Material and methods. The blood concentrations of tDNA, fetal DNA (fDNA) and maternal DNA (mDNA) were determined in women at 11-14, 24-26, and 30-32 weeks. The level of tDNA was estimated by quantitative PCR through determination of the concentration of the RASSF1A gene and that of fDNA was calculated through determination of the hypermethylated RASSF1A gene. Results. The concentration of extracellular tDNA and mDNA did not change substantially during pregnancy. Until mid-gestation, there was a relatively stable fDNA level (averaged 1265.11+577.51 GE/ml at 11-14 weeks and 1309.09+561.01 GE/ml at 24-26 weeks) and its considerable increase in the second half of pregnancy. The level of extracellular DNA and its fractions did not change in relation to the women’s BMI and fetal and placental weight. In anemia, the concentration of mDNA averaged 12362.33+5533.22 GE/ml and was significantly lower than in healthy pregnant women (an average of 15268.49+5973.43) (p < 0.05). Conclusion. The f indings conf irm the change in the concentration of total extracellular DNA during pregnancy, which must be taken into account when predicting its complications. To eliminate the effects of other maternal and fetal factors, it is necessary to continue investigations.

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About the authors

Anna Ovikovna Karapetyan

National Medical Research Center of Obstetrics, Gynecology, and Perinatologynamed after Academician V.I. Kulakov

Email: anne-89@mail.ru
post-graduate student

Alexey Mikhailovich Krasnyi

National Medical Research Center of Obstetrics, Gynecology, and Perinatologynamed after Academician V.I. Kulakov

Email: a_krasnyi@oparina4.ru
Ph.D., head. Laboratory of Cytology

Alsu Amirovna Sadekova

National Medical Research Center of Obstetrics, Gynecology, and Perinatologynamed after Academician V.I. Kulakov

Email: a_sadekova@oparina4.ru
Researcher, Laboratory of Cytology

Galina Vladimirovna Khlestova

National Medical Research Center of Obstetrics, Gynecology, and Perinatologynamed after Academician V.I. Kulakov

Email: g_khlestova@oparina4.ru
post-graduate student

Ivan Sergeevich Balashov

National Medical Research Center of Obstetrics, Gynecology, and Perinatologynamed after Academician V.I. Kulakov

Email: i_balashov@oparina4.ru
Junior Researcher, Laboratory of Bioinformatics

Oleg Radomirovich Baev

National Medical Research Center of Obstetrics, Gynecology, and Perinatologynamed after Academician V.I. Kulakov; I.M. Sechenov First Moscow State Medical University

Email: o_baev@oparina4.ru
MD, professor, head of the maternity department

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