The role of E-kadherin in the formation of intrauterine growth restriction


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Abstract

Objective. To study the relationship between levels of soluble (80-kd) and transmembrane (120-kd) forms of E-kadherin in placentas from pregnancies with intrauterine growth restriction. Materials and methods. The study was done on 38 placentas: 18 from pregnancies with intrauterine growth restriction (main group) and 20 from normal pregnancies (control group). Levels of soluble and transmembrane E-kadherin in placentas were determined by Western blotting. Measurement of E-kadherin gene expression were made by real-time polymerase chain reaction. Results. In the group with intrauterine growth restriction the level of the transmembrane form of E-cadherin was reduced by 2.5 times, and the soluble form by 4.8 times (p <0.05). Their ratio was of 1.6 in the group with intrauterine growth restriction,and of - 0.8 in control group (in both cases p <0.05). The level of expression of the E-cadherin gene CDH1 in the placentas from pregnancies with fetal growth restriction was reduced by 1.3 times as compared to the control group (p> 0.05). Conclusion. The low level of soluble E-cadherin in the placenta leads to a decrease in proliferation and mobility of trophoblast cells, which may be the cause of placental insufficiency and, as a consequence, intrauterine growth restriction.

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About the authors

Aleksey M. Krasnyi

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia; N.K. Koltzov Institute of Developmental Biology of Russian Academy of Sciences

Email: alexred@list.ru
PhD, the head of the cytology laboratory; senior researcher of evolutionary developmental biology laboratory N.K. Koltzov

Anuta A. Khachaturyan

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: x.anyt37@mail.ru
postgraduate student

Natalia E. Kan

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: kan-med@mail.ru
PhD, MD, the head of the obstetric department

Zarine V. Khachatryan

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: z.v.khachatryan@gmail.com
postgraduate student

Victor L. Tyutyunnik

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: tioutiounnik@mail.ru
PhD, MD, the head of the obstetric physiological department

Nadezda E. Volgina

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: n_volgina@oparina4.ru
scientific researcher of the cytology laboratory

Maria B. Ganichkina

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: mariaganichkina@yandex.ru
postgraduate student

Diana A. Mantrova

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: d-mantrova@yandex.ru
postgraduate student

Alsu A. Sadekova

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: a_sadekova@oparina4.ru
scientific researcher of the cytology laboratory

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