Assessment of microRNA expression in retrocervical endometriotic lesions


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Abstract

Aim. To profile microRNA in the tissues of the ectopic and eutopic endometrium and identify signaling pathways and processes regulated by differentially expressed microRNAs. Material and methods. Differentially expressed microRNAs were detected using the next-generation sequencing technique. The sequencing data were validated by real-time polymerase chain reaction. Signaling pathways and processes were estimated by enriching information databases with bioinformatic analysis. Results. RNA sequencing resulted in the identification of 429 miRNAs with different patterns in the tissues of the eutopic and ectopic endometrium. As a result of statistical analysis based on the reliability of differences and representation in all comparison groups, 85 miRNAs were selected to determine the possible involvement of their target genes in intracellular cascades and the processes regulated by them. Bioinformatic analysis showed the involvement of regulated target genes into the processes of proliferation, migration, invasion, and into key cascades of intracellular signaling and inflammatory processes. Conclusion. The findings suggest that the change in the microRNA expression in the ectopic endometrium and subsequent disruption of the regulation of the expression of their target genes is an important pathogenic link and may contribute to the development of endometriosis.

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About the authors

Mikhail Y. Bobrov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: mbobr@mail.ru
Ph.D., Head of the Laboratory of molecular pathophysiology

Ivan S. Balashov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: i_balashov@oparina4.ru
Expert, the Bioinformatics research group

Elena S. Filippova

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

obstetrician-gynecologist of the gynecological department

Indira K. Almova

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: www.gold11@mail.ru
postgraduate student of the Surgery Department

Anzhelika V. Timofeeva

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: v_timofeeva@oparina4.ru
Ph.D., Senior Researcher, Laboratory of Molecular Pathophysiology

Vladislava A. Gusar

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: v_gusar@oparina4.ru
Ph.D., Senior Researcher, Laboratory of Molecular Pathophysiology

Pavel I. Borovikov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: p_borovikov@oparina4.ru
Head of the Bioinformatics research group

Elena G. Khilkevich

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: e_khilkevich@oparina4.ru
MD, obstetrician-gynecologist of the Surgery Department

Vladimir D. Chuprynin

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: v_chuprynin@oparina4.ru
PhD, Head of the Surgery Department

Stanislav V. Pavlovich

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia

Email: st.pavlovich@mail.ru
Ph.D., Associate Professor, Scientific Secretary; Professor of the Department of Obstetrics, Gynecology, Perinatology, and Reproductology, Institute of Professional Education

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