The role of RIG-I, AiM2 and IFI16 receptors for viral RNA and DNA in the pathogenesis of spontaneous and early missed miscarria
- Autores: Lebedeva O.P.1, Zhukova I.O.1, Ivashova O.N.1, Pakhomov S.P.1, Churnosov M.I.1
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Afiliações:
- Belgorod State National Research University
- Edição: Nº 7 (2018)
- Páginas: 57-61
- Seção: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248295
- DOI: https://doi.org/10.18565/aig.2018.7.57-61
- ID: 248295
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Resumo
Aim. To identify the features of the expression of mRNA of intracellular RIG-I, IFI16 and AIM2 receptors for viral RNA and DNA, and their signaling pathway proteins in the decidual tissue of patients with spontaneous and early missed miscarriages. Material and methods. The study comprised 68 patients with sporadic miscarriages, including 34 women with missed and 34 with spontaneous miscarriages at 6 to10 weeks of gestation. The control group included 57 patients who underwent surgical termination of pregnancy at the same gestational age. Patients with severe non-obstetric comorbidities, antiphospholipid syndrome, endocrine causes of miscarriage, and confirmed fetal chromosomal abnormalities were excluded from the study. Decidual tissues were collected by uterine curettage. Expression of RIG-I, IFI16 and AIM2 mRNA and their signaling pathway proteins (ASC, TBK1, STING, caspase-1, interleukin-1ß) was analyzed using quantitative polymerase chain reaction. Results. Patients with missed miscarriages had a seven-fold increase in the expression of decidual AIM2 mRNA protein detecting intracellular viral and Listeria’s DNA. This elevation was accompanied by an increase in the caspase-1 and IL-1β mRNA expression resulting in the initiation of an inflammatory response and pyroptotic cell death. Patients with missed miscarriages did not have significant changes in the expression of RIG-I and IFI16 mRNA, and their signaling pathway proteins. No changes in AIM2, RIG-I, and IFI16 mRNA expression and their signaling pathway proteins were observed in patients with spontaneous miscarriage. Conclusion. Patients with missed miscarriages have an increased decidual expression of the AIM2 receptor for viral and Listeria’s DNA. This increase is accompanied by an increase in the caspase-1 and IL-1β mRNA expression, leading to the initiation of a pro-inflammatory response and cell death.
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Sobre autores
Olga Lebedeva
Belgorod State National Research University
Email: safonova2@yandex.ru
MD, associate professor, professor of the Department of Obstetrics and Gynaecolgy
Irina Zhukova
Belgorod State National Research University
Email: ronny413@mail.ru
resident of the Department of Obstetrics and Gynaecology
Olesya Ivashova
Belgorod State National Research University
Email: ivashovao2@yandex.ru
assistant of the Department of Obstetrics and Gynaecology
Sergey Pakhomov
Belgorod State National Research University
Email: pachomw@yandex.ru
MD, professor, Head of the Department of Obstetrics and Gynaecology
Mikhail Churnosov
Belgorod State National Research University
Email: churnosov@bsu.edu.ru
MD, professor, Head of the Department of Medical and Biological Sciences
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