Features of the expression of the immunohistochemical markers P16 and KI67 in women with chronic cervicitis associated with bacterial and viral infections
- Authors: Bokach O.M.1, Niauri D.A.2, Tishkov A.V.3, Selkov S.A.3
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Affiliations:
- Family Planning Center “Medica”
- St. Petersburg State University
- D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- Issue: No 9 (2018)
- Pages: 85-90
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248330
- DOI: https://doi.org/10.18565/aig.2018.9.85-90
- ID: 248330
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Abstract
Objective. To investigate the features of the expression of P16 and Ki67 in women with an abnormal colposcopic pattern and chronic cervicitis associated with bacterial and viral infections. Material and methods. The materials were genitourinary discharge and cervical biopsy samples from women diagnosed with chronic cervicitis. The investigators made microscopic examination of Gram stained smears from the urethra, vagina, and cervical canal, as well as culture and RT-PCR assay of cervicovaginal secretions, cervical cancer cytology, and p16 and Кі-67 histological and immunohistochemical studies of cervical tissues. Results. In women with chronic cervicitis, the association of high oncogenic risk human papillomavirus (HPV) with Chlamydia trachomatis and Mycoplasma genitalium was found to increase the frequency of Ki- 67 expression in two thirds of the cervical epithelium by 4 times as compared to that of high oncogenic risk HPV with Mycoplasma hominis and/or Ureaplasma urealyticum. Conclusion. When the abnormal colposcopic pattern is detected in women with chronic cervicitis, a histological study of cervical tissues should be complemented with Ki-67 expression examination for the differential diagnosis of the severity of cervical neoplasia.
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About the authors
Olga M. Bokach
Family Planning Center “Medica”
Email: Olga.bokach634@yandex.ru
obstetrician-gynecologist
Dariko Aleksandrovna Niauri
St. Petersburg State University
Email: d.niauri@mail.ru
professor, head of Department of Obstetrics, Gynecology and Reproductology of the medical faculty
Artem Valerievich Tishkov
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: artem.tishkov@gmail.com
associate professor, head of Physics, Mathematics and Informatics Department
Sergey A. Selkov
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: selkovsa@mail.ru
professor, head of the Department of Immunology and Intercellular Interactions
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