Assessment of expression of stem cell markers in eutopic and ectopic endometrium in women with ovarian endometriotic cysts


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Abstract

Objective. To compare levels of PDGFR and CD15 in eutopic, ectopic and normal endometrium. Materials and methods. Group I included 70 patients with ovarian endometriotic cysts, and Group II comprised 24 patients with unexplained infertility without endometriosis. Endoscopic removal of endometriotic cyst and hysteroscopy were performed in women in Group I; laparoscopy, chromohydrotubation and hysteroscopy with material sampling for immunohistochemistry were performed in Group II. Results. An increase in CD15 expression in the ectopic endometrium was observed both in the gland (35(32;37), 8 (8;9), p = 0.002) and in stroma (16(15;22), 1(0.8;1.3), p = 0.003); an increase in PDGFR was also detected both in the gland (38(38;41), 9(8; 10), p = 0.002), and in stroma 18(17; 19), 1.1(0.8;1.4), p = 0.001). We noted an increase in CD15 (8(8;9), 8(6.5;8), p = 0.006) and PDGFR (9(8;10), 8(7.5;9), p = 0.001) in the glands of the eutopic endometrium in comparison with the normal one. Conclusion. The results obtained during the study confirm the theory of the role of stem cells in the pathogenesis of endometriosis, which may underlie the development of pathogenetic therapy for this disease.

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About the authors

Svetlana O. Dubrovina

Rostov State Medical University

Email: s.dubrovina@gmail.com
MD, professor, main researcher, Research Institute of Obstetrics and Pediatrics

Yuliya D. Berlim

Rostov State Medical University

Email: juliaberlim@yandex.ru
PhD, the head of the Outpatient Department

Vadim S. Chirsky

Email: v_chirsky@mail.ru
MD, professor, head of the Central Pathological Anatomy Laboratory, Ministry of Defense of the Russian Federation, chief pathologist of the Ministry of Defense of the Russian Federation.

Vladimir Yu. Mazhugin

Rostov State Medical University

Email: 79604623623@yandex.ru
head of the Laboratory of Pathological Morphology, Research Institute of Obstetrics and Pediatrics

Knarik A. Areshyan

Rostov State Medical University

Email: arieshian@bk.ru
postgraduate student, Research Institute of Obstetrics and Pediatrics

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