Increasing the effectiveness of IVF programs by determining mitochondrial DNA copy number in embryonic trophectoderm


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Abstract

Objective. To improve the effectiveness of in vitro fertilization (IVF) programs in patients of late reproductive age by transferring euploid embryos with normal levels of mtDNA. Material and methods. This pilot study analyzed the copy number of mitochondrial DNA (mtDNA) in trophectoderm cells of 106 embryos obtained from 50 married couples. After blastocyst trophectoderm biopsy and preimplantation genetic testing for aneuploidy (PGT-A) by a chip-based comparative genomic hybridization (aCGH), the mtDNA copy number was determined using real-time polymerase chain reaction (RT-PCR). Results. The mtDNA copy number in the trophectoderm of aneuploid embryos (n = 38) was statistically significantly higher than in the trophectoderm of euploid blastocysts (n = 68) (p = 0.003). The ROC analysis resulted in the development of an mtDNA quantity threshold of 0.004 r.u., which was predictive for implantation failure of euploid embryos with above-threshold mtDNA levels with a76.8% sensitivity and 74.9% specificity. Conclusion. Determining the mtDNA copy number in trophectoderm cells of blastocysts in women undergoing PGT-A is a reliable method for selecting embryos with high implantation potential, which implies the possibility of increasing the effectiveness of infertility treatment in IVF programs.

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About the authors

Anna I. Korolkova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: zaikinaai@icloud.com
PhD, junior researcher, 1st gynecology Department of Gynecology

Nona G. Mishieva

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: nondoc555@mail.ru
MD, senior researcher, 1st Department of Gynecology

Bella A. Martazanova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: bellamart88@mail.ru
PhD, junior researcher, 1st Department of Gynecology

Olga V. Bourmenskaya

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: o_bourmenskaya@oparina4.ru
MD, researcher, Laboratory of Molecular Genetics

Aleksey N. Ekimov

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: a_ekimov@oparina4.ru
MD, geneticist, Laboratory of Molecular Genetics

Dmitry Y. Trofimov

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: d_troflmov@oparina4.ru
MD, head of the Laboratory of Molecular Genetics

Maria A. Veyukova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: veymary@gmail.com
PhD, embryologist, 1st Department of Gynecology

Anastasia O. Kirillova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: a_kozyreva@oparina.ru
PhD, embryologist,lst Department of Gynecology

Aidar N. Abubakirov

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov Ministry of Health of Russia

Email: nondoc555@yahoo.com
PhD, head of the 1st Department of Gynecology

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