Molecular biological features of HPV type 16 infection and the risk of developing cervical squamous intraepithelial lesions and cervical cancer


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Abstract

Objective. To determine the prevalence of HPV type 16 variants in the Russian Federation (Moscow and Moscow region) and investigate the association between mutations of human papillomavirus type 16 in the long control region (LCR), E6 and E7 genes and the probability of detecting high-grade CIN and cervical cancer. Material and methods. The study examined 63 cervical epithelial scrape samples positive for HPV type 16 that was confirmed by histological and cytological evidence. Results. Sixty-two of 63 samples belonged to lineage A, and only one sample was assigned to lineage D. Six substitutions for reference sequences were detected, of which four were in the LCR (G7193T, A7316C, T7496C, C24G), and 2 in the E6gene (T109C, T350G). C24G substitution was statistically significantly more common in samples from CIN2-3 and cervical cancer lesions (OR = 2.41, p = 0.04). Conclusions. This study for the first time reported the prevalence of HPV type 16 variants in the Russian Federation (Moscow and the Moscow Region) and showed that the population of HPV type 16 is quite monogenic and represented by the European lineage. Six substitutions were found in the examined fragment, and one of them (C24G) was statistically significantly more common in samples from CIN+ lesions. Substitutions A7316C, T109C and T350G showed a trend towards higher detection rates in samples showing CIN2-3.

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About the authors

Marina Yu. Dmitryukova

Researcher at the CRI of Epidemiology of Rospotrebnadzor

Email: mdmitryukova@cmd.su
PhD (bio.sci.)

Lyubov’ I. Korolenkova

Blokhin Russian Cancer Research Center of Minzdrav of Russia

Email: l.korolenkova@mail.ru
MD, professor, senior researcher

Tat’yana N. Romanyuk

CRI of Epidemiology of Rospotrebnadzor

Email: tatiana.romaniuk@pcr.ms
junior researcher

Gul’nara V. Leshkina

CRI of Epidemiology of Rospotrebnadzor

Email: glyoshkina@yandex.ru
biologist

Ol’ga Yu. Shipulina

CRI of Epidemiology of Rospotrebnadzor

Email: olga.shipulina@pcr.ms
PhD, research team leader

German A. Shipulin

CRI of Epidemiology of Rospotrebnadzor

Email: german@pcr.ru
Ph.D., Head of the department of Molecular Diagnostics and Epidemiology

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