Complete zona pellucida removal from blastocysts prior to transfer in a cryo-cycle does not affect the efficiency of in vitro fertilization programs


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To evaluate the efficiency of in vitro fertilization (IVF) programs during the transfer of blastocysts in a cryo-cycle with their zona pellucida (ZP) removal. Material and methods. An experimental group included 209 patients whose embryos before transfer were subjected to complete mechanical ZP removal; a control group consisted of222patients, in whom blastocysts with intact ZP were used for transfer. The rates of embryo implantation and clinical and progressive clinical pregnancy were estimated. Results. All clinical outcomes of the programs had no statistically significant differences in the group of embryos with the removed ZP and with the embryos in the control group, including subgroups of patients younger and older than 36 years of age. Conclusion. Complete zona pellucida removal from blastocysts prior to their transfer in a cryo-cycle does not affect the efficiency of IVFprograms.

Full Text

Restricted Access

About the authors

Konstantin Vladimirovich Kirienko

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: kkiriyenko@rambler.ru

Valentina Petrovna Apryshko

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"; M.V. Lomonosov Moscow State University

Email: supermycolog@mail.ru

Anna Gennadievna Mironova

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"; N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Margarita Aleksandrovna Kharitonova

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: ritasarc@mail.ru

Irina Yurievna Ermilova

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: i_ermilova@altravita.ru

Anna Aleksandrovna Naumova

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: naumo.ann@yandex.ru

Alexey Aleksandrovich Klepukov

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: klepukov@altravita.ru

Anton Igorevich Bolt

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: a_bolt@altravita.ru

Iana Vadimovna Bozina

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: yana.bozina@gmail.com

Elena Borisovna Lebedeva

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"

Email: lebedester@gmail.com

Ekaterina Yurevna Simonenko

M.V. Lomonosov Moscow State University

Email: ksimonenko@inbox.ru

Sergey Aleksandrovich Yakovenko

AltraVita Clinic of Human Reproduction, OOO "ECO CENTER"; M.V. Lomonosov Moscow State University

Email: altravita@mail.ru

References

  1. Fujiwara K., Sano D., Seita Y., Inomata T., Ito J., Kashiwazaki N. Ethylene glycol-supplemented calcium-free media improve zona penetration of vitrified rat oocytes by sperm cells. J. Reprod. Dev. 2010; 56(1): 169-75. https://doi. org/10.1262/jrd.09-107H.
  2. Hiraoka K., Fuchiwaki M., Hiraoka K., Horiuchi T., Murakami T., Kinutani M. et al. Re-cryopreservation by vitrification of human blastocysts developed from frozencleaved embryos: a report of 15 cycles. J. Mammalian Ova Res. 2007; 24(1): 23-8. https://doi.org/10.1274/jmor.24.23.
  3. Martins W.P., Rocha I.A., Ferriani R.A., Nastri C.O. Assisted hatching of human embryos: a systematic review and meta-analysis of randomized controlled trials. Hum. Reprod. Update. 2011; 17(4): 438-53. https://doi.org/10.1093/ humupd/dmr012.
  4. Vajta G., Rienzi L., Bavister B.D. Zona-free embryo culture: is it a viable option to improve pregnancy rates? Reprod. Biomed. Online. 2010; 21: 17-25. https:// doi.org/10.1016/j.rbmo.2010.03.014.
  5. Hiraoka K., Fuchiwaki M., Horiuchi T., Murakami T., Kinutani M., Kinutani K. Zona pellucida removal and vitrified blastocyst transfer outcome: a preliminary study. Reprod. Biomed. Online. 2007; 15(1): 68-75. https://doi.org/10.1016/ S1472-6483(10)60694-3.
  6. Yakovenko S., Apryshko V., Yutkin E., Troshina M., Simonenko E. Technique and effectiveness of intracytoplasmic morphologically selected sperm injection (IMSI) based on HMC (Hoffman modulation contrast). Fertil. Steril. 2009; 92( 3, Suppl.): S162. (Scientific Abstracts from the 2009 Annual Meeting of the American Society for Reproductive Medicine.)
  7. Yakovenko S. IMSI Hoffman modulation. In: International Workshop on Male Infertility - IMSI: new advances. June 24-27, 2010 Rome, Italy.
  8. Jelinkova L., Pavelkova J., Strehler E., Paulus W, Zivny J., Sterzik K. Improved implantation rate after chemical removal of the zona pellucida. Fertil. Steril. 2003; 79(6): 1299-303. https://doi.org/10.1016/S0015-0282(03)00260-7.
  9. Kinget K., Nijs M., Cox A.M., Janssen M., Jacobs P., Bosmans E. et al. A novel approach for patients at risk for ovarian hyperstimulation syndrome, elective transfer of a single zona-free blastocyst on day 5. Reprod. Biomed. Online. 2002; 4: 51-5. https://doi.org/10.1016/S1472-6483(10)61915-3.
  10. Isik A.Z, Vicdan K., Kaba A., Dagli G. Comparison of zona manipulated and zona intact blastocyst transfers, a prospective randomized trial. J. Assist. Reprod. Genet. 2000; 17(3): 135-9. https://doi.org/10.1023/A:1009410020580.
  11. Urman B., Balaban B., Alatas C., Aksoy S., Mumcu A., Isiklar A. Zona-intact versus zona-free blastocyst transfer, a prospective, randomized study. Fertil. Steril. 2002; 78(2): 392-6. https://doi.org/10.1016/S0015-0282(02)03238-7.
  12. Mansour R.T., Rhodes C.A., Aboulghar M.A., Serour G.I., Kamal A. Transfer of zona-free embryos improves outcome in poor prognosis patients, a prospective randomized controlled study. Hum. Reprod. 2000; 15(5): 1061-4. https://doi. org/10.1093/humrep/15.5.1061.
  13. Lan K.C., Huang F.J., Lin Y.C., Kung F.T., Chang S.Y. Zona-free versus laser zona-assisted hatching blastocyst transfer, a comparison of outcomes. Fertil. Steril. 2009; 91(5): 1959-62. https://doi.org/10.1016/j.fertnstert.2008.01.068.
  14. Hiraoka K., Fuchiwaki M., Hiraoka K., Horiuchi T., Murakami T., Kinutani M. et al. Zona pellucida removal and vitrified blastocyst transfer outcome, a preliminary study. Reprod. Biomed. Online. 2007; 15(1): 68-75.
  15. Carney S.K., Das S., Blake D., Farquhar C., Seif M.M., Nelson L. Assisted hatching on assisted conception in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Cochrane Database Syst. Rev. 2012; (12): CD001894. https://doi.org/10.1002/14651858.CD001894.pub5.
  16. Li D., Yang D.L., An J., Jiao J., Zhou Y.M., Wu Q.J. et al. Effect of assisted hatching on pregnancy outcomes, a systematic review and meta-analysis of randomized controlled trials. Sci. Rep. 2016; 6: 31228. https://doi.org/10.1038/ srep31228.
  17. Ueno S., Ezoe K., Yabuuchi A., Uchiyama K., Okimura T., Okuno T. et al. Complete zona pellucida removal from vitrified-warmed human blastocysts facilitates earlier in-vitro attachment and outgrowth. Reprod. Biomed. Online. 2016; 33(2): 140-8. https://doi.org/10.1016/j.rbmo.2016.05.007.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2019 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies