FEATURES OF THE PROFILE OF PROTEINS SECRETED BY CELLS FROM THE ENDOMETRIOID FOCI AND EUTOPIC ENDOMETRIUM IN WOMEN WITH EXTERNAL GENITAL ENDOMETRIOSIS IN IN VITRO CULTURE


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Abstract

Objective. To comparatively assess the profile of proteins secreted in vitro culture by cells from endometrioid foci and eutopic endometrium of women with external genital endometriosis. Material and methods. The cells isolated from the samples of endometrioid heterotopias and eutopic endometrium from 21 women with external genital endometriosis and the endometrial cells from 8 women without endometriosis were cultured in vitro. The surface phenotype of cells taken after Passage 1 was determined using a flow cytometer and a standard set of monoclonal antibodies. During Passage 2, a conditioned culture medium was used to estimate the concentration of various soluble analytes by a multiplexed analysis. Results. The cells from the endometrioid foci and eutopic endometrium, which were cultured before Passage 2, adhered to plastic, showed f ibroblast-like morphology, and phenotypically met the minimum criteria adopted for the identification of mesenchymal stromal cells. The culture supernatants displayed a different level of cell production of the test proteins from the foci of endometriosis and eutopic endometrium. Conclusion. In vitro production of a wide range of proteins characterizing the functional state of stromal cells isolated from the ectopic foci of endometriosis indicates the increased cell activation in the endometrioid foci, which is an important pathogenetic factor in the development of endometriosis.

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About the authors

V. Vladislavovich PavlovichStanislav

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia

Email: s_pavlovich@oparina4.ru
M.D., Ph. D. - Academic secretary 117997, Russia, Moscow, Ac. Oparina str. 4

L. V Krechetova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: l_krechetova@oparina4.ru
Ph.D. in medical sciences, head of clinical immunology laboratory 117997, Russia, Moscow, Ac. Oparina str. 4

Valentina V. Vtorushina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_vtorushina@oparina4.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory 117997, Russia, Moscow, Ac. Oparina str. 4

L. V Vanko

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: LVanko@oparina4.ru
Doctor of Medicine, professor, leading research worker in Laboratory of clinical immunology 117997, Russia, Moscow, Ac. Oparina str. 4

M. N Yushina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

researcher, laboratory of molecular pathophysiology Ostrovitianov str. l, Moscow, Russia, ll7997

Anastasia M. Savilova

N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia

Email: lsavilova@gmail.com
Ph.D., Head of Information and Analytical Center 117997, Russia, Moscow, Ac. Oparina str. 4

N. Nikolaevich MakiyanZograb

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: z_makiyan@oparina4.ru
Doctor of medicine, principal scientist, working in the department of operative gynecology 117997, Russia, Moscow, Ac. Oparina str. 4

Ekaterina L. Yarotskaya

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_yarotskaya@oparina4.ru
MD, Associate Professor, Head of the Department of International Cooperation 117997, Russia, Moscow, Ac. Oparina str. 4

Elena G. Khilkevich

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_khilkevich@oparina4.ru
MD, obstetrician-gynecologist of the Surgery Department 117997, Russia, Moscow, Ac. Oparina str. 4

Vladimir D. Chuprynin

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_chuprynin@oparina4.ru
PhD, Head of the Surgery Department 117997, Russia, Moscow, Ac. Oparina str. 4

Gennady T. Sykhikh

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia

Email: e_khilkevich@oparina4.ru
MD, Professor, Academical of the Russian Academy of Sciences, Director 117997, Russia, Moscow, Ac. Oparina str. 4

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