Diagnosis of vulvar condyloma acuminatum: clinical and molecular genetic aspects


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Abstract

Objective. To optimize the diagnosis of vulvar condyloma acuminatum (CA), by expanding the possibilities of using the polymerase chain reaction (PCR) in clinical practice. Subjects and methods. Ninety patients with vulvar CA were examined. All the examined women underwent vulvoscopy with a vinegar test, high-frequency ultrasound of the skin and vulvar mucosa, human papillomavirus (HPV) typing in the samples of the cervical canal epithelium, scrapes from CA, and its biopsy specimens. Biopsies (n = 90) were performed in AC measuring more than 3 mm in diameter. Result. Analyzing the identification of HPV types revealed that HPV 6 and HPV 44 were statistically significantly more common in the biopsy specimens of CA than in those of the cervical canal and in the CA scrapes; HPV 11 was found statistically significantly more frequently in the scrapes from CA than in those from the cervical canal and in the biopsy specimens. High-risk HPV types were also detected in the CA biopsy specimens, while the viral load was more often found to be statistically significantly higher in the samples of the cervical canal epithelium. The viral load in the scrapes from CA on the vulvar mucosa turned out to be statistically significantly higher than that in those on the vulvar skin. Conclusion. CAs on the vulvar mucosa, even with small sizes, are a reservoir of HPV infection and have a more pronounced viral potential than those located on the skin, therefore CAs absolutely require timely and effective treatment.

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About the authors

Inna A. Apolikhina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; I.M. Sechenov First Moscow State Medical University

Email: apolikhina@inbox.ru
MD, Professor, Head of aesthetical gynecology and rehabilitation department Moscow, Russia

Gulnar F. Gasanova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: ice8507@rambler.ru
graduent student Moscow, Russia

Dmitriy Y. Trofimov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: d_troflmov@oparina4.ru
MD, Professor, Head of clinical and molecular genetics Department Moscow, Russia

Andrey E. Donnikov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: a_donnikov@oparina4.ru
PhD, Head of Laboratory of molecular-genetic methods Moscow, Russia

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