Assessment of Ki67 immunohistochemical expression as a prognostic marker in breast carcinoma


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Abstract

Aim. To compare Ki-67 proliferation marker expression in unspecified type of breast cancer determined by traditional visual assessment and digital microscopy using PatternQuant software (QuantCenter 3DHISTECH). Material and methods. A comparative study including 50 patients with unspecified type of breast cancer was conducted to compare the determination of the Ki-67 proliferation marker by a pathologist using the traditional visual quantitative assessment and by digital microscopy using PatternQuant software (QuantCenter 3DHISTECH). Results. Despite comparable mean Ki-67 expressions, their maximum and minimum values determined by a pathologist were slightly higher than by automated assessment. This is especially important in determining a threshold for Ki-67 expression (14%) in each individual case to distinguish molecular subtypes of breast cancer. Conclusion To objectify the quantitative assessment, a further standardized examination of breast cancer proliferation markers is recommended, especially in cases of significant tumor heterogeneity.

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About the authors

Larisa V. Volkova

Immanuel Kant Baltic Federal University

Email: volkova-lr@rambler.ru

Fedor N. Paramzin

Central City Clinical Hospital, Kaliningrad

Email: fedia93@gmail.com

Aleksander I. Pashov

Immanuel Kant Baltic Federal University

Email: pachov@mail.ru

Aleksey A. Musatov

Immanuel Kant Baltic Federal University

Email: sayaleks@gmail.com

Lev A. Ashrafyan

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology of Minzdrav of Russia

Email: evaa2004@yahoo.com

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