Methylation of the TLR2 and IGF2/H19 ICR genes in the placenta and blood plasma in preeclampsia


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Abstract

Objective. To study the methylation profile of genes in the placenta and plasma of patients with preeclampsia. Materials and methods. The study included two groups of pregnant women. The main group consisted of 26 women with preeclampsia, 12 of them had moderate preeclampsia and 14 women had severe preeclampsia. The control group consisted of 26 women with normal pregnancy. The methylation level of 22 genes was determined. The study was conducted using Methylation-Sensitive High Resolution Melting curve analysis (MS-HRM). Results. The study of methylation of IGF2/H19 imprinting control region (ICR) showed a statistically significant decrease in the relative level of methylation in the placenta and blood plasma in patients with moderate and severe preeclampsia (p<0.05). There was a statistically significant increase in the level of the TLR2 gene methylation in the placenta and blood plasma in patients with severe preeclampsia in comparison with the patients with normal pregnancy (p<0.05). Conclusion. The results of the study indicate that the TLR2gene methylation and the IGF2/H19 ICR influence the development of a systemic inflammatory response in preeclampsia. The level of gene methylation in blood plasma correlates with the level of genes in the placenta and can be used as early non-invasive markers of preeclampsia.

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About the authors

Daiana A. Boris

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: dayana_boris@mail.ru
postgraduate student

Aleksey N\\. Krasnyi

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: alexred@list.ru
PhD, the Head of the cytology laboratory

Sergey V. Kurevlev

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: s_kurevlev@oparina4.ru
scientific researcher of the cytology laboratory

Alsu A. Sadekova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_sadekova@oparina4.ru
PhD, scientific researcher of the cytology laboratory

Natalia E. Kan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; European Medical Center

Email: bn-med@mail.ru
PhD, MD, head doctor

Victor L. Tyutyunnik

European Medical Center

Email: tioutiounnik@mail.ru
PhD, MD, deputy head doctor

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