Lipid markers of metastatic lesions in regional lymph nodes in patients with breast cancer


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Abstract

Aim. To assess the possibility of diagnosis of lymph node metastasis of breast cancer by lipid profile in normal and malignant breast tissue. Materials and methods. Semiquantitative evaluation of the lipids in tissue was performed using HPLC-MS/MS of tissue organic extracts. The lipids were identified by accurate molecular masses and tandem mass-spectra (MS/ MS). To create the logistic regression based on the Akaike information criteria, the lipids with significantly lipid level difference were used (p<0.05by Mann-Witney U-test). Results. The obtained diagnostic models based on normal tissue had sensitivity 81% and specificity 79%, the diagnostic model based on malignant tissue had sensitivity 78% and specif icity 81 % respectively. The lipids, which were selected as markers of lymph node metastases, belonged to sphingomyelins, ether lipids, phosphatidylcholines and phosphotidylethanoamines. Conclusion. The study confirmed that ether lipids and sphingomyelins may be the indicators of metastasis and demonstrated the possibility to use them for metastasis diagnosis.

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About the authors

Alisa O. Tokareva

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation; V.L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Center of Chemical Physics; Moscow Institute of Physics and Technology

Email: alisa.tokareva@phystech.edu
specialist of the Laboratory of Proteomics and Metobolomics of the Human Reproduction

Vitaliy V. Chagovets

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation

Email: vvchagovets@gmail.ru
Ph.D. in Physical and Mathematical Sciences, Senior researcher of the Laboratory of Proteomics and Metobolomics of the Human Reproduction

Valeriy V. Rodionov

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation

Email: V_Rodionov@oparina4.ru
M.D., Head of the Department of Breast Pathology

Vlada V. Kometova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation

Email: v_kometova@oparina4.ru
PhD in Medical Sciences, Senior researcher of the Anatomical Pathology Department

Maria V. Rodionova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation

Email: m_Rodionova@oparina4.ru
PhD in Medical Sciences, Oncologist of the Department of Breast Pathology

Natalia L. Starodubtseva

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation; V.L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Center of Chemical Physics

Email: aurum19@mail.ru
PhD in Biological Sciences, Head of the Laboratory of Proteomics and Metobolomics of the Human Reproduction

Vladimir E. Frankevich

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation

Email: v_frankevich@oparina4.ru
Ph.D. in Physical and Mathematical Sciences, Systems Biology in Reproduction

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