Steroid hormone profiles in reproductive age patients with advanced endometriosis


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Abstract

Introduction. Investigating pathological changes in endogenous steroidogenesis associated with advanced extragenital endometriosis (AEGE) requires high-precision and sensitive methods. Aim. To investigate blood steroidome changes in AEGE. Materials and methods. Patients with AEGE and control subjects were tested for serum hormones using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Results. Patients with AEGE had significant changes in the concentration of 9 steroids. Blood hormone concentrations in patients with and without AEGE were as follows: estradiol 0.18 vs. 0.12 ng/ml (p=0.05), 17-OH-progesterone0.55vs. 0.44ng/ml(p=0.05), testosterone0.81 vs. 0.43ng/ml(p<0.01), dihydrotestosterone- 0.16 vs. 0.37 ng/ml (p<0.1), dehydroepiandrosterone 3.56 vs. 5.98 ng/ml (p<0.01), DHEA sulfate 2533.00 vs. 4425.00 ng/ml (p<0.01), androstenedione 1.35 vs. 1.31 ng/ml (p=0.29), cortisol 164.00 vs. 90.50 ng/ml (p<0.01), corticosterone 4.87 vs. 1.20 ng/ml (p<0.01), 11-deoxycorticosterone 0.87 vs. 0.37 ng/ml (p<0.01). Progesterone concentrations were below the detection limit in both groups. Four logistic regression models allowed differentiation of blood steroidome of AEGE patients from that in controls. Conclusion. Endometriotic cysts in stage III - IVAEGE have no significant effect on changes in the steroidome. HPLC-MS differences in blood steroidome allow differentiation of patients with AEGE from control subjects.

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About the authors

Stanislav V. Pavlovich

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: s_pavlovich@oparina4.ru
MD, Academic Secretary

Mariia V. Iurova

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: m_yurova@oparina4.ru
Specialist

Vitaliy V. Chagovets

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: vvchagovets@gmail.com
Ph.D., Senior Researcher at the Laboratory of Proteomics and Metabolomics in Human Reproduction, Department of System Biology

Vladimir E. Frankevich

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_frankevich@oparina4.ru
Ph.D., Head of Department of System Biology in Reproduction

Nataliya L. Starodubtseva

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_starodubtseva@oparina4.ru
Ph.D., Head of Laboratory of Proteomics of Human Reproduction

V. D Chuprynin

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_chuprynin@oparina4.ru

Gennady T. Sukhikh

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: g_sukhikh@oparina4.ru
MD, Academician of the Russian Academy of Sciences, Director

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