MICROBIOTA OF PRETERM INFANTS BORN TO MOTHERS WITH ENDOCRINE DISORDERS


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Abstract

Aim. The study aimed to investigate differences in the microbiota composition of newborn infants from mothers with and without endocrine disorders. Materials and methods. We collected 143 stool samples from preterm infants born to mothers with or without endocrine disorders. Samples were taken from the first stool passed by the newborn (meconium) one week and two weeks after birth. DNA isolated from the samples underwent sequencing of the V3-V4 region of the 16S rRNA gene. Taxonomic profiles were assessed using the DADA2. The search for differentially represented bacterial genera was carried out using the DESeq2 package. Results. Newborn infants from mothers with and without endocrine disorders had a significantly different composition of the gut microbiota. Differences started to emerge one week after birth. Infants from mothers with endocrine disorders had increased relative abundance of opportunistic microorganisms and reduced relative abundance of genera Bifidobacterium and Lactobacillus. Conclusion. Our findings of differences in preterm infant gut microbiota composition suggest that the maternal endocrine system’s state can influence the infant gut microbiome’s formation during the early stages of its colonization.

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About the authors

Tatiana V. Priputnevich

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: priput1@gmail.com
Dr. Med. Sci., Director of the Institute of Microbiology, Antimicrobial Therapy and Epidemiology 4, Oparin str., Moscow, 117997, Russia

Anastasia V. Nikolaeva

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_nikolaeva@oparina4.ru
Ph.D., Chief physician 4, Oparin str., Moscow, 117997, Russia

Natalia E. Shabanova

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_shabanova@oparina4.ru
Ph.D., Associate Professor, Researcher at the Unit of Clinical Pharmacology, Institute of microbiology, Antimicrobial Therapy and Epidemiology 4, Oparin str., Moscow, 117997, Russia

Dmitry E. Fedorov

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: fedorov.de@gmail.com
Research Laboratory Assistant at the Laboratory of Genome Research and Computational Biology 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

Alexander I. Manolov

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: paraslonic@gmail.com
Researcher at the Laboratory of Genome Research and Computational Biology 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

Alexander V. Pavlenko

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: pavav@mail.ru
Researcher at the Laboratory of Genome Research and Computational Biology 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

Dmitry N. Konanov

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: konanovdmitriy@gmail.com
Laboratory Research at the Laboratory of Genome Research and Computational Biology 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

Danil V. Krivonos

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: danil01060106@gmail.com
Student at the Laboratory of Genome Research and Computational Biology 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

Ksenia M. Klimina

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: ppp843@yandex.ru
Senior Researcher at the Laboratory of Genome Research and Computational Biology 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

Vladimir A. Veselovskiy

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: djdf26@gmail.com
Junior Researcher at the Laboratory of Genome Research and Computational Biology 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

Viktor V. Zubkov

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: victor.zubkov@mail.ru
Dr. Med. Sci., Professor, Director of the Institute of Neonatology and Pediatrics 4, Oparin str., Moscow, 117997, Russia

Elena N. Il’ina

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: ilinaen@gmail.com
Corr. Member of the RAS, Dr. Bio. Sci., Deputy Director for Science, Head of the Department of Molecular Biology and Genetics 1A Malaya Pirogovskaya str., Moscow, 119435, Russia

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