Analysis of heparan sulfate proteoglucans content in eutopic and heterotopic endometrium in ovarian endometriosis


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Abstract

Aim. To analyze the content of heparan sulfate proteoglycans (HSPGs) in eutopic and heterotopic endometrium in women with ovarian endometriosis (OE). Materials and methods. Surgical samples of endometrioid ovarian cysts and endometrial biopsy specimens obtained from 11 women of reproductive-age in the proliferative phase of the ovarian cycle with stage III OE (according to the American Fertility Society classification) were analyzed using real-time semi-quantitative PCR and immunohistochemistry (IHC). The expression patterns of core proteins of three HSPGs and the enzyme heparanase (HPSE) were assessed. Nonparametric statistical methods were used for data processing. Results. Real-time PCR analysis of the relative expression of core proteins of HSPGs in endometrial heterotopias revealed a 2-fold increase in total transcriptional activity of the gene encoding perlecan (HSPG2) and HPSE compared to eutopic endometrium (p<0,05). IHC analysis of these molecules in surgical samples confirmed these data. The relative expression of HPSE in heterotopias positively correlated with concentration of the tumor marker CA125 in blood serum. Conclusion. HSPG2 and HPSE participate in the pathomorphogenesis of OE, leading to extracellular matrix modification and impairment of intercellular communications. They can be used as markers of stage III OE.

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About the authors

Yulia S. Timofeeva

Novosibirsk State Medical University, Ministry of Health of Russia

Email: yustimofeeva@gmail.com
assistent of the Obstetrics and Gynecology Department

Dmitriy K. Sokotov

Scientific Research Institute of Molecular Biology and Biophysics of Federal Research Centre of Fundamental and Translational Medicine, Ministry of Science and Higher Education of the Russian Feeration

Email: dmit_s95@mail.ru
postgraduate student, Laboratory of Glycobiology

Elvira V. Grigorieva

Scientific Research Institute of Molecular Biology and Biophysics of Federal Research Centre of Fundamental and Translational Medicine, Ministry of Science and Higher Education of the Russian Feeration

Email: elv_grig@mail.ru
PhD, Head of Laboratory of Glycobiology

Alexander V. Volchek

Novosibirsk State Medical University, Ministry of Health of Russia

Email: alexander@volcheck.ru
junior researcher

Galina M. Kazanskaya

Scientific Research Institute of Molecular Biology and Biophysics of Federal Research Centre of Fundamental and Translational Medicine, Ministry of Science and Higher Education of the Russian Feeration

Email: g_kazanskaya@meshalkin.ru
PhD, researcher of Laboratory of Glycobiology

Anastasiya V. Sukhovskikh

Scientific Research Institute of Molecular Biology and Biophysics of Federal Research Centre of Fundamental and Translational Medicine, Ministry of Science and Higher Education of the Russian Feeration

PhD, researcher of Laboratory of Glycobiology

Alexandra Yu. Tsidulko

Scientific Research Institute of Molecular Biology and Biophysics of Federal Research Centre of Fundamental and Translational Medicine, Ministry of Science and Higher Education of the Russian Feeration

researcher of Laboratory of Glycobiology

Yanina M. Evseeva

Novosibirsk State Medical University, Ministry of Health of Russia

Email: janinaevseeva@yahoo.com
student

Vitaliy M. Kuleshov

Novosibirsk State Medical University, Ministry of Health of Russia

MD, professor, professor of the Obstetrics and Gynecology Department

Igor O. Marinkin

Novosibirsk State Medical University, Ministry of Health of Russia

MD, professor, Head of the Obstetrics and Gynecology Department

Svetlana V. Aidagulova

Novosibirsk State Medical University, Ministry of Health of Russia

Email: s.aydagulova@gmail.com
PhD, professor, Head of Laboratory of Cellular Biology and Fundamental Basis of Reproduction

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