Analysis of vitamin D receptor expression in patients with genital endometriosis


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Abstract

Aim. To evaluate expression of vitamin D receptors (VDR) in endometriotic heterotopies and endometrium of patients with genital endometriosis (GE). Materials and methods. 52 women aged 31.8 (4.3) years were included in the study. The main group consisted of 32patients with surgically and histologically confirmed GE of stages I-IVaccording to the r-ASRM classification. The control group included 20 women who were examined for infertility and in whom no pelvic disease was found at diagnostic laparoscopy. The expression of VDR in endometriotic lesions and in endometrium of patients with GE as well as in endometrium of the patients from the control group was evaluated. Qualitative and quantitative evaluation of the results of immunohistochemical study was performed using digital microscopy and morphometry. Excel, Statistica 10 and Jamovi programs were used for results processing. Results. Endometriotic heterotopies are characterized by decreased values of VDR AE in comparison with both proliferative and secretory endometrium of patients with GE, and secretory endometrium from women of the control group. We did not find cyclic changes in VDR expression in endometrium of patients with GE compared to endometrium of the control group. Conclusion. The results of study elucidate the role of vitamin D in pathogenesis of endometriosis and give us the opportunity to justify the use of cholecalciferol as a prom ising therapy for this disease.

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About the authors

Maria I. Yarmolinskaya

D.O.Ott Research Institute of Obstetrics, Gynecology and Reproductology; I.I. Mechnikov North-Western State Medical University, Ministry of Health of the Russian Federation

Email: m.yarmolinskaya@gmail.com
Professor of the Russian Academy of Sciences, Dr. Med. Sci., Professor, Head of the Department of Gynecology and Endocrinology, Head of the center "Diagnostics and treatment of endometriosis”

Alexandra S. Denisova

D.O.Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: al.ser.denisova@gmail.com
post-graduate student, junior researcher of the Department of Gynecology and Endocrinology

Gulrukhsor Kh. Tolibova

D.O.Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: gulyatolibova@yandex.ru
Dr. Med. Sci., Head of the Laboratory of Immunohistochemistry, Head of the Laboratory of Morphology

Olesya N. Bespalova

D.O.Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: shiggerra@mail.ru
Dr. Med. Sci., Deputy Director for Research

Tatyana G. Tral

D.O.Ott Research Institute of Obstetrics, Gynecology and Reproductology; Saint Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation

Email: ttg.tral@yandex.ru
PhD, senior researcher of the Laboratory of Morphology, Head of the Department of Pathologic Anatomy

Karina A. Zakuraeva

D.O.Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: kareenz@yandex.ru
clinical resident

Valeria O. Pyankova

D.O.Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: pyankova.valeriia@rambler.ru
post-graduate student

References

  1. Barnett R., Banks N., Decherney A.H. Endometriosis and fertility preservation. Clin. Obstet. Gynecol. 2017; 60(3): 517-23. https://dx.doi.org/10.1097/ GRF.0000000000000311.
  2. Parasar P., Ozcan P., Terry K.L. Endometriosis: epidemiology, diagnosis and clinical management. Curr. Obstet. Gynecol. Rep. 2017; 6(1): 34-41. https:// dx.doi.org/10.1007/s13669-017-0187-1.
  3. Brosens I., Brosens J., Benagiano G. Neonatal uterine bleeding as antecedent of pelvic endometriosis. Hum. Reprod. 2013; 28(11): 2893-7. https://dx.doi. org/10.1093/humrep/det359.
  4. Ярмолинская М.И., Айламазян Э.К. Генитальный эндометриоз. Различные грани проблемы. СПб.: Эко-Вектор; 2017. 615 с.
  5. Muscogiuri G., Altieri B., de Angelis C., Palomba S., Pivonello R., Colao A. et al. Shedding new light on female fertility: The role of vitamin D. Rev. Endocr. Metab. Disord. 2017; 18(3): 273-83. https://dx.doi.org/10.1007/s11154-017-9407-2.
  6. Денисова А. С., Ярмолинская М.И. Роль витамина D. в патогенезе генитального эндометриоза. Журнал акушерства и женских болезней. 2017; 66(6): 81-8. [Denisova A.S., Yarmolinskaya M.I. The role of vitamin D. in the pathogenesis of genital endometriosis. Zhurnal akusherstva i zhenskih boleznej / Journal of obstetrics and women's diseases. 2017; 66(6): 81-8. (in Russian)]. https://doi.org/10.17816/J0WD66681-88.
  7. Cermisoni G.C., Alteri A., Corti L., Rabellotti E., Papaleo E., Vigano P., Sanchez A.M. Vitamin D. and endometrium: a systematic review of a neglected area of research. Int. J. Mol. Sci. 2018; 19(8): 2320. https://dx.doi.org/10.3390/ ijms19082320.
  8. Nandi A., Sinha N., Ong E., Sonmez H., Poretsky L. Is there a role for vitamin D. in human reproduction? Horm. Mol. Biol. Clin. Investig. 2016; 25(1): 15-28. https://dx.doi.org/10.1515/hmbci-2015-0051.
  9. Мальцев С.В., Мансурова Г.Ш. Метаболизм витамина D. и пути реализации его основных функций. Практическая медицина. 2014; 9: 12-8.
  10. Суслова Е.В., Ярмолинская М.И., Ткаченко Н.Н., Клейменова Т.С., Нетреба Е.А. Значение пролактина, дофамина и их рецепторного профиля в развитии генитального эндометриоза. Проблемы репродукции. 2019; 25(6): 86-94. [Suslova E.V., Yarmolinskaya M.I., Tkachenko N.N., Klejmenova T.S., Netreba E.A. The role of prolactin, dopamine and their receptor profile in the development of genital endometriosis. Problemy reprodukcii/Russian Journal of Human Reproduction. 2019; 25(6): 86-94. (in Russian)]. https://doi. org/10.17116/repro20192506186.
  11. Ярмолинская М.И., Тхазаплижева С.Ш., Молотков А. С., Ткаченко Н.Н., Бородина В.Л., Андреева Н.Ю., Клейменова Т.С., Лысенко В.В. Мелатонин и наружный генитальный эндометриоз: роль в патогенезе и возможности применения в терапии заболевания. Журнал акушерства и женских болезней. 2019; 68(3): 51-60. [Yarmolinskaya M.I., Thazaplizheva S.Sh., Molotkov A.S., Tkachenko N.N., Borodina V.L., Andreeva N.Yu. et al. Melatonin and external genital endometriosis: role in the pathogenesis and possible use in the treatment of the disease. Zhurnal akusherstva i zhenskih boleznej/ Journal of obstetrics and women's diseases. 2019; 68(3): 51-60 (in Russian)]. https:// dx.doi.org/10.17816/JOWD68351-60.
  12. Толибова Г.Х., Траль Т.Г., Клещёв М.А., Кветной И.М., Айламазян Э.К. Эндометриальная дисфункция: алгоритм гистологического и иммуноги-стохимического исследования. Журнал акушерства и женских болезней. 2015; 64(4): 69-77.
  13. Zelenko Z., Aghajanova L., Irwin J.C., Giudice L.C. Nuclear receptor, coregulator signaling, and chromatin remodeling pathways suggest involvement of the epigenome in the steroid hormone response of endometrium and abnormalities in endometriosis. Reprod. Sci. 2012; 19(2): 152-62. https://dx.doi. org/10.1177/1933719111415546.
  14. Bergada L., Pallares J., Arcidiacono M.V., Cardus A., Santacana M., Valls J. et al. Role of local bioactivation of vitamin D. by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab. Invest. 2014; 94(6): 608-22. https://dx.doi.org/10.1038/labinvest.2014.57.
  15. Agic A., Xu H., Altgassen C., Noack F., Wolfler M.M., Diedrich K. et al. Relative expression of 1,25-dihydroxyvitamin D3 receptor, vitamin D. 1 alpha-hydroxylase, vitamin D. 24-hydroxylase, and vitamin D. 25-hydroxylase in endometriosis and gynecologic cancers. Reprod. Sci. 2007; 14(5): 486-97. https://dx.doi.org/10.1177/1933719107304565.
  16. Harmon Q.E., Kissell K., Jukic A.M.Z., Kim K., Sjaarda L., Perkins N.J. et al. Vitamin D. and reproductive hormones across the menstrual cycle. Hum. Reprod. 2020; 35(2): 413-23. https://dx.doi.org/10.1093/humrep/dez283.
  17. Monastra G., De Grazia S., De Luca L., Vittorio S., Unfer V. Vitamin D: a steroid hormone with progesterone-like activity. Eur. Rev. Med. Pharmacol. Sci. 2018; 22(8): 2502-12.
  18. Адамян Л.В., Сонова М.М., Тихонова Е.С., Зимина Э.В., Антонова С.О. Медицинские и социальные аспекты генитального эндометриоза. Проблемы репродукции. 2011; 17(6): 78-81.
  19. Бахарева И.В. Витамин D. и эндометриоз: в поиске новых возможностей. Российский вестник акушера-гинеколога. 2018; 18(4): 35-43.
  20. Mariani M., Vigano P., Gentilini D., Camisa B., Caporizzo E., Di Lucia P. et al. The selective vitamin D. receptor agonist, elocalcitol, reduces endometriosis development in a mouse model by inhibiting peritoneal inflammation. Hum. Reprod. 2012; 27(7): 2010-19. https://dx.doi.org/10.1093/humrep/ des150.
  21. Abbas M.A., Taha M.O., Disi A.M., Shomaf M. Regression of endometrial implants treated with vitamin D3 in a rat model of endometriosis. Eur. J. Pharmacol. 2013; 715(1-3): 72-5. https://dx.doi.org/10.1016/j.ejphar.2013.06.016.
  22. Yildirim B., Guler T., Akbulut M., Oztekin O., Sariiz G. 1-Alpha, 25- Dihydroxyvitamin D3 regresses endometriotic implants in rats by inhibiting neovascularization and altering regulation of matrix metalloproteinase. Postgrad. Med. 2014; 126(1): 104-10. https://dx.doi.org/10.3810/ pgm.2014.01.2730.
  23. Ярмолинская М.И., Денисова А.С., Андреева Н.Ю. Эффективность применения витамина D3 (колекальциферола) в терапии хирургически индуцированного эндометриоза у крыс. Российский вестник акушера-гинеколога. 2019; 19(3): 37-42. https:/dx./doi. org/10.17116/rosakuch20191903137.

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