The role of interleukin-8 (IL-8) and polymorphism of IL-8 gene in formation of external genital endometriosis in patients of reproductive age


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Abstract

Aim. To study IL-8 production and IL-8 (251T>A) gene polymorphism in patients of reproductive age with external genital endometriosis (EGE). Materials and methods. The study included 71 patients with different stages of external genital endometriosis and 24 patients without endometriosis in the control group. The levels of IL-8 in serum and peritoneal fluid were determined by enzyme immunoassay using the kits produced by MedSystems (Austria). The distribution of alleles and genotypes of the 251T>A polymorphism of the gene IL-8 was carried out by the method of polymerase chain reaction followed by restriction analysis (GosNIIgenetika, Moscow). Results. The increased IL-8 concentrations in the peritoneal fluid were found in patients with stages I-IIEGE. The distribution of IL-8 gene allelic variants among patients with various stages of ЕGE was characterized by a predominance of the T/T genotypes of the 251T> A polymorphism. Conclusion. Increased IL-8 concentrations in the peritoneal fluid and the presence of the polymorphic marker 251T>A in IL-8 gene were associated with a risk of development of EGE in patients of reproductive age.

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About the authors

Tatevik G. Avanesova

City Hospital №8, Rostov-on-Don

Email: tata_avanesova@mail.ru
Ph.D., obstetrician-gynecologist

Marina A. Levkovich

Rostov State Medical University, Ministry of Health of Russia, Research Institute of Obstetrics and Pediatrics

Email: xlma@mail.ru
Dr. Med. Sci., Associate Professor, Leading Researcher at the Department of Medical and Biological Problems in Obstetrics, Gynecology and Pediatrics

Natalia V. Ermolova

Rostov State Medical University, Ministry of Health of Russia, Research Institute of Obstetrics and Pediatrics

Email: rniiap.ermolova@gmail.com
Dr. Med. Sci., Associate Professor, Head of the Department of Obstetrics and Gynecology

Natalia V. Palieva

Rostov State Medical University, Ministry of Health of Russia, Research Institute of Obstetrics and Pediatrics

Email: nat-palieva@yandex.ru
Dr. Med. Sci., Professor at the Department of Obstet-rics and Gynecology No2, Leading Researcher at the Department of Obstetrics and Gynecology

Yuriy A. Petrov

Rostov State Medical University, Ministry of Health of Russia

Email: mr.doktorpetrov@mail.ru
Dr. Med., Sci., professor, Head of the Department of Obstetrics and Gynecology No2

References

  1. Адамян Л.В., Фархат К.Н., Макиян З.Н., Савилова А.М. Молекулярнобиологическая характеристика эутопического эндометрия (обзор литературы). Проблемы репродукции. 2015; 21(5): 8-16. [Adamyan L.V., Farkhat K.N., Makiyan Z.N., Savilova A.M. The external genital endometriosis: theories and molecular investigations (a review). Problemy reproduktsii/ Problems of Reproduction. 2015; 21(5): 8-16 (in Russian)]. https://dx.doi. org/10.17116/repro20152158-16.
  2. Коган Е.А., Акопова Е.О., Унанян А.Л. Бесплодие при эндометриозе: краткий очерк современных представлений. Пространство и время. 2017; 1: 251-9.
  3. Ермолова Н.В., Друккер Н.А., Погорелова Т.Н., Слесарева К.В., Томай Л.Р., Маркарьян И.В., Трушина С.А. Молекулярно-биологические технологии в диагностике и дифференцированном подходе к лечению эндометриоза. Журнал акушерства и женских болезней. 2016; 65 (Приложение): 8-10.
  4. Wimberger P., Grubling N., Riehn A., Furch M., Klengel J., Goeckenjan M. Endometriosis - a chameleon: patients’ perception of clinical symptoms, treatment strategies and their impact on symptoms. Geburtshilfe Frauenheilkd. 2014; 74(10): 940-6. https://dx.doi.org/10.1055/ s-0034-1383168.
  5. Rogers P.A.W., Adamson G.D., Al-Jefout M., Becker C.M., DHooghe T.M., Dunselman G.A.J. et al. Research priorities for endometriosis. Reprod. Sci. 2017; 24(2): 202-26. https://dx.doi.org/10.1177/1933719116654991.
  6. Ярмолинская М.И., Зайцев Д.В., Тхазаплижева С.Ш. Мелатонин и генитальный эндометриоз - новые возможности терапии. Журнал акушерства и женских болезней. 2015; 64(1): 67-75. [Yarmolinskaya M. I., Zaytsev D. V., Tkhazaplizheva S. Sh. Melatonin and genital endometriosis - new possibilities for therapy. Zhurnal akusherstva i zhenskikh boleznei/Journal of Obstetrics and Womans Diseases. 2015; 64(1): 67-75. (in Russian)]. https://dx.doi. org/10.17816/J0WD64167-75.
  7. Yeo S.G., Won Y.S., Lee H.Y., Kim Y.I., Lee J.W., Park D.C. Increased expression of pattern recognition receptors and nitric oxide synthase in patients with endometriosis. Int. J. Med. Sci. 2013; 10(9): 1199-208. https://dx.doi. org/10.7150/ijms.5169.
  8. Brosens I., Puttemans P., Benagiano G. Endometriosis: a life cycle approach? Am. J. Obstet. Gynecol. 2013; 209(4): 307-16. https://dx.doi.org/10.1016/j. ajog.2013.03.009.
  9. Acien P., Velasco I. Endometriosis: a disease that remains enigmatic. ISRN Obstet. Gynecol. 2013; 2013: 242149. https://dx.doi.org/10.1155/2013/242149.
  10. Сонова М.М., Адамян Л.В., Жорданиа К.И., Паяниди Ю.Г. Эндометриоз и рак яичников. Есть ли взаимосвязь? Общие патогенетические черты рака яичников и эндометриоза. Онкогинекология. 2013; 4: 30-40.
  11. Левкович М.А., Ермолова Н.В., Аванесова Т.Г. Иммунопатогенез генитального эндометриоза. Российский аллергологический журнал. 2017; 14 (Приложение 1): 76-8.
  12. Паяниди Ю.Г., Жорданиа К.И., Логинов В.И., Чемерис Г.Ю., Сивакова Н.Г. Эндометриоз и эндометриоидный рак яичников. Акушерство и гинекология: новости, мнения, обучение. 2017; 1: 44-8. [Payanidi Yu.G., Zhordania K.I., Loginov V.I., Chemeris G.Yu., Sivakova N.G. Endometriosis and endometrioid ovarian cancer. Akusherstvo i ginekologiya: novosti, mneniya, obuchenie/Obstetrics and gynecology: news, opinions, training. 2017; 1: 44-8. (in Russian)]. https://dx.doi.org/10.24411/2303-9698-2017-00016.
  13. Melin A., Lundholm C., Malki N., Swahn M.L., Sparen P., Bergqvist A. Endometriosis as a prognostic factor for cancer survival. Int. J. Cancer. 2011; 129(4): 948-55. https://dx.doi.org/10.1002/ijc.25718.
  14. Nassif J., Mattar S., Abu Musa A., Eid A. Endometriosis and cancer: what do we know? Minerva Ginecol. 2013; 65(2): 167-79.
  15. Асташкин E.,И., Кречетова Л.В., Ванько Л.В. Роль нейтрофильных гранулоцитов в развитии эндометриоза. Акушерство и гинекология. 2020; 6: 5-12. [Astashkin E.I., Vanko L.V., Krechetova L.V. The role of neutrophilic granulocytes in the development of endometriosis. Obstetrics and Gynecology. 2020; 6: 5-12. (in Russian)]. https://dx.doi.org/10.18565/aig.2020.6.5-12.
  16. Сельков С.А., Ярмолинская М.И. Эндометриоз как патология регуляторных механизмов. Журнал акушерства и женских болезней. 2017; 66(2): 9-13. [Selkov S.A., Yarmolinskaya M.I. Endometriosis as a pathology of regulatory mechanisms. Zhurnal akusherstva i zhenskikh boleznei/Journal of Obstetrics and Womans Diseases. 2017; 66(2): 9-13. (in Russian)]. https:// dx.doi.org/10.17816/jowd6629-13.
  17. Рзаева А.Ш. Распространение полиморфизма интерлейкина-8 - 251 ТА среди женщин Азербайджана больными эндометриозом. Успехи современного естествознания. 2011; 3: 32-4.
  18. Si kora J., Smycz-Kubanska M., Mielczarek-Palacz A., Kondera-Anasz Z. Abnormal peritoneal regulation of chemokine activation - the role of IL-8 in pathogenesis of endometriosis. Am. J. Reprod. Immunol. 2017; 77(4): e12622. https://dx.doi.org/10.1111/aji.12622.
  19. Красильникова А.К., Малышкина А.И., Сотникова Н.Ю., Анциферова Ю.С. Особенности функционального состояния фагоцитов крови у пациенток с эндометриозом I-II стадии и бесплодием. Российский вестник акушера-гинеколога. 2017; 17(3): 9-14. [Krasilnikova A.K., Malyshkina A.I., Sotnikova N.Yu., Antsiferova Yu.S. The functional state of phagocytes in patients with stage I-II endometriosis and infertility. Rossiiskii vestnik akushera-ginekologa/ Russian Bulletin of Obstetrician-Gynecologist. 2017; 17(3): 9-14. (in Russian)]. https://dx.doi.org/10.17116/rosakush20171739-14.
  20. Питиримова Л.Н., Загороднева Е.А., Гумилевский Б.Ю. Особенности аллельного полиморфизма генов интерлейкинов и цитокиновый баланс женщин с невынашиванием беременности. Акушерство и гинекология. 2014; 3: 33-8.
  21. Левкович М.А., Ермолова Н.В., Аванесова Т.Г., Маркарьян И.В. Современные взгляды на патогенез генитального эндометриоза: роль гормональных, иммунологических, генетических факторов. Таврический медико-биологический вестник. 2017; 20(2-2): 185-9.
  22. Ярмолинская М.И., Айламазян Э.К., Сельков С.А. Иммунологические аспекты генитального эндометриоза. В. кн.: Репьева Н.Н., ред. Генитальный эндометриоз. Различные грани проблемы. СПб.: ЭКО-Вектор; 2017: 12673.
  23. Lagana A.S., Sturlese E., Retto G., Sofo V., Triolo O. Interplay between misplaced Mullerian-derived stem cells and peritoneal immune dysregulation in the pathogenesis of endometriosis. Obstet. Gynecol. Int. 2013; 2013: 527041. https://dx.doi.org/10.1155/2013/527041.

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