Influence of controlled mechanical microvibration on embryo metabolomic profile


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Abstract

Objective. To assess the influence of controlled mechanical microvibration (CMMV) on the metabolomic profile of the 5-day embryo culture media (CM). Materials and methods. This was a prospective cohort study of 62 CM samples collected from 44 patients. CM samples were obtained during IVF cycles on the fifth day of embryo development: 20 embryos were cultivated under CMMV conditions (microvibration group), and 42 embryos were cultivated under standard conditions (control group). In the microvibration group, the incubator was placed on an ArisTT180-s platform (K&S Advanced Systems Ltd, Israel) in an active vibration mode at a frequency of 40 Hz for 30 seconds with intervals of 30 minutes. The metabolites were extracted by adding three volumes of methanol and subsequently centrifuged. Chromatographic separation was performed using a reverse-phase chromatographic system on an Atlantis T3 C18 column (Waters, USA). Metabolites were detected on a hybrid quadrupole-time-of-flight mass spectrometer (Bruker Daltoniks, Germany). Results. The analysis revealed significant differences in the metabolite profiles between the groups. The most significant changes were in regulatory molecules (progesterone, acetylcholine, oleamide, prostaglandin A2 and its glutathione conjugate, 2,3-dinor-thromboxane B2 and 20-hydroxy prostaglandin E2), amino acids and their metabolites (glutamine, hydroxypropyl glutamate, lysyl-gamma-glutamate). Conclusions. There is a significant influence of controlled mechanical microvibration on the profile of metabolites in the culture media of the 5-day embryos. Further research should be conducted to analyze the impact of these differences on pregnancy rate, its course, and perinatal outcomes.

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About the authors

Andrey Yu. Romanov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia

Email: romanov1553@yandex.ru
MD, Researcher of R&D Department

Chupalav M. Eldarov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia

Ph.D. in Chemistry, Senior Researcher at the Laboratory of Molecular Pathophysiology

Alexandra M. Frolova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia

Email: i.a.m.frolova@mail.ru
embryologist of IVF Department

Nataliya P. Makarova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia

Email: np_makarova@oparina4.ru
PhD, Leading Researcher of IVF Department

Mikhail Yu. Bobrov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia

Email: mbobr@mail.ru
Ph.D. in Chemistry, Head of the Laboratory of Molecular Pathophysiology

Nataliya V. Dolgushina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of Russia

Email: n_dolgushina@oparina4.ru
M.D., Ph.D., M.P.H., Head of R&D Department

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