PLACENTA-SPECIFIC MICRO-RNA EXPRESSION IN FETAL GROWTH RESTRICTION


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Abstract

Objective. To analyze the expression of placenta-specific microRNAs in pregnant women with fetal growth restriction (FGR) and with physiological pregnancy. Materials and methods. The investigation enrolled 42 women at 30-34 weeks’ gestation (27 with FGR and 15 with uncomplicated pregnancy), who were matched for age, anthropometric characteristics, features of an obstetric and gynecological history, and concomitant diseases. All the pregnant women with FGR were observed to have manifestations of different degrees of fetoplacental insufficiency. The patients underwent peripheral venous blood sampling. The expression of eight microRNAs (microRNA- 10b-5p, microRNA-145-5p, microRNA-122-5p, microRNA-141-3p, microRNA-125b-5p, microRNA-205-5p, microRNA-210-3p, and microRNA-517-5p) was determined by real-time polymerase chain reaction (PCR). The change in microRNA expression levels was estimated using the AST method. Results. It was ascertained that the expression of microRNA-125b-5p in the blood of pregnant women with FGR was statistically significantly lower (12.3 (8.9; 13.8)) than that in women with physiological pregnancy (8.1 (6.6; 9.1)) (p=0.011). The magnitude of expression change (fold change) for microRNA-125b-5p was 5.25. Conclusion. The findings indicate a change in the microRNA-125b-5p expression levels in the pregnant women with FGR versus the women with uncomplicated pregnancy. The role of micro-RNA-125b-5p in the genesis of FGR can be explained by the ability of this microRNA to affect the vascular endothelium, including the placental vasculature, disrupting the processes of endothelium-dependent relaxation, angiogenesis, endothelial cell proliferation, and other functions, Including the regulation of platelet adhesion and aggregation.

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About the authors

Irina O. Bushtyreva

"Professor Bushtyreva Clinic" LLC

Email: kio4@mail.ru
Dr. Med. Sci., Full Professor 58/7A Sobornyi ave., Rostov-on-Don, 344010, Russia

Natalia B. Kuznetsova

Rostov State Medical University, Ministry of Health of the Russian Federation; "Professor Bushtyreva Clinic" LLC

Email: lauranb@inbox.ru
Dr. Med. Sci., Professor at the Department of Simulation education 29 Nahichevansky ave., Rostov-on-Don, 344022, Russia

Ekaterina A. Zabanova

Rostov State Medical University, Ministry of Health of the Russian Federation; Perinatal Center

Email: rock-fe@mail.ru
Postgraduate at the Department of Simulation education 29 Nahichevansky ave., Rostov-on-Don, 344022, Russia

Elena V. Butenko

South Federal University

Email: evbutenko@sfedu.ru
Cand. Bio. Sci., Associate Professor at the Department of Genetics 194/1 Stachki ave., Rostov-on-Don, 344090, Russia

Inna O. Pokudina

South Federal University

Email: ipokudina@sfedu.ru
Cand. Bio. Sci., Senior researcher at the Laboratory of Biomedicine 194/1 Stachki ave., Rostov-on-Don, 344090, Russia

Tatiana P. Shkurat

South Federal University

Email: tshkurat@sfedu.ru
Dr. Bio. Sci., Full Professor, Director of Research Institute of Biology 194/1 Stachki ave., Rostov-on-Don, 344090, Russia

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