Non-invasive testing of human preimplantation embryos in vitro as a way to predict the outcomes of in vitro fertilization programs


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Abstract

The data available in the current literature on non-invasive methods for diagnosing the quality of an embryo and its genetic status were systematically analyzed. The review includes data from foreign and Russian articles published in Pubmed on this topic over the past 3 years. The improvement in the outcome of the IVF program is determined by many factors, including the quality of an embryo. The expansion of knowledge in relation to its development and physiology largely due to various current data processing methods and technologies can determine not only the level of embryonic morphological development, but also to predict the potential for further development. The non-invasiveness, safety, and efficiency of the method are the main criteria for current diagnosis of the potential of the embryo. Numerous studies of the embryo culture medium meet these requirements and seem promising in the selection of high-quality embryos. Conclusion. The study of the molecular composition of culture media for the embryo makes it possible to comprehensively consider its life cycle, to assess the relationship of cellular metabolism to deep regulatory mechanisms. The development of omix technologies could gain insights into the molecular profile of the embryo culture medium, by identifying and characterizing the biomarkers that are potentially important for the onset of pregnancy. Further improvement of methods for analyzing culture media, processing the data, and increasing the future scope of research can provide a new, non-invasive predictor for the quality of the embryo and its implantation potential.

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About the authors

E. Z Valiakhmetova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: ibraeva1988@list.ru
postgraduate student at the Department of Assisted Reproductive Technologies in the Treatment of Infertility 117997, Russia, Moscow, Ac. Oparina str., 4

E. V Kulakova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: e_kulakova@oparina4.ru
Ph.D. in physics and mathematics, Director 117997, Russia, Moscow, Ac. Oparina str., 4

Yu. S. Skibina

Research and Production Enterprise "Nanostructured Glass Technology" and International Research and Education Center "Structure-Mediated al Nanobiophotonics"

Email: director@nano-glass.ru
Ph.D. in physics and mathematics, Director 410033, Russia, Saratov, pr. 50 let Octyabrya, 101

A. Yu Gryaznov

Research and Production Enterprise "Nanostructured Glass Technology" and International Research and Education Center "Structure-Mediated al Nanobiophotonics"

Email: director@nano-glass.ru
Researcher 410033, Russia, Saratov, pr. 50 let Octyabrya, 101

A. P Sysoeva

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: a_sysoeva@oparina4.ru
embryologist at the Department of Assisted Reproductive Technologies in the Treatment of Infertility 117997, Russia, Moscow, Ac. Oparina str., 4

N. P Makarova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: np_makarova@oparina4.ru
Dr. Bio. Sci., Leading Researcher at the Department of Assisted Reproductive Technologies in the Treatment of Infertility 117997, Russia, Moscow, Ac. Oparina str., 4

E. A Kalinina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: e_kalinina@oparina4.ru
Dr. Med. Sci., Professor, Head of the Department of Assisted Reproductive Technologies in the Treatment of Infertility 117997, Russia, Moscow, Ac. Oparina str., 4

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