A new method of multimodal optical coherence tomography for the diagnosis of vulvar lichen sclerosus


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Abstract

Objective. To investigate whether an intravital multimodal optical coherence tomography (OCT) technique can be used to diagnose vulvar lichen sclerosus. Materials and methods. The investigation enrolled 6 gynecology unit patients who were divided into 2 groups according to histological criteria for lichen sclerosus: 1) patients without signs of vulvar mucosal damage (a control group) and 2) those who had the late stage of lichen sclerosus (a study group). Immediately before a biopsy, the mucosal condition at the points studied was evaluated by multimodal OCT angiography and OCT lymphangiography. Results. Multimodal OCT demonstrated decreases in the thickness of the epithelium, in the contrast of the epithelial/connective tissue border, and in the density of blood and lymphatic vessels compared to the intact mucosa. These changes reflect the criteria for the histological classification of lichen sclerosus. Conclusion. The OCT signs lichen sclerosus were demonstrated to correspond with its histological features. OCT was shown to be a promising in vivo tool for the diagnosis of sclerotic lichen.

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About the authors

Arseniy L. Potapov

Volga Research Medical University

Email: arseniyll09@gmail.com
student

Elizaveta A. Konovalova

Volga Research Medical University

Email: konovalova.7@gmail.com
student

Marina A. Sirotkina

Volga Research Medical University

Email: sirotkina_m@mail.ru
Ph.D., Head of Laboratory of High Resolution Microscopy and Gene Technologies, Research Institute of Experimental Oncology and Biomedical Technologies

Naylya N. Vagapova

N.A. Semashko Nizhny Novgorod Regional Clinical Hospital

Email: vagapova_nelly@mail.ru
gynecologist of the Ist gynecological department

Ivan K. Safonov

Volga Research Medical University

Email: iv.safonov20l7@yandex.ru
Clinical Resident

Anna A. Timakova

I.M. Sechenov First Moscow State Medical University (Sechenov University), Russian Ministry of Health

Email: atimakova96@gmail.com
student

Stefka G. Radenska-Lopovok

I.M. Sechenov First Moscow State Medical University (Sechenov University), Russian Ministry of Health

Email: radenska@mail.ru
MD, Professor, A.I. Strukov Department of Pathological Anatomy

Sergey S. Kuznetsov

I.M. Sechenov First Moscow State Medical University (Sechenov University), Russian Ministry of Health

Email: zunek@mail.ru
MD, Professor, Head of Pathological and Anatomical Department

Elena V. Zagainova

Volga Research Medical University

Email: ezagaynova@gmail.com
MD, Professor of RAS, Director of Research Institute of Experimental Oncology and Biomedical Technologies

Irina A. Kuznetsova

Volga Research Medical University

Email: irakuz2007@mail.ru
MD, Professor, Head of Pathological and Anatomical Department

Natal’ya D. Gladkova

Volga Research Medical University

Email: natalia.gladkova@gmail.com
MD, Professor, Head of Laboratory of Optical Coherent Tomography, Scientific Research Institute of Experimental Oncology and Biomedical Technologies

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