Phenotypic characterization of peripheral blood innate immune cell subpopulations in women with endometriosis before and after surgery


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Abstract

Objective: To investigate phenotypic characteristics of innate immune cell subpopulations in women with endometriosis before and after combination therapy, including surgery. Materials and methods: The study included women with stage I-II (n=12) and III-IV(n=28) endometriosis, who underwent clinical and immunological investigations before and after combination therapy, including surgery. Peritoneal fluid samples obtained intraoperatively and blood samples drawn from the study subjects before and six months after surgery were phenotyped for mononuclear cells using flow cytometry (n=15). Results: In the peritoneal fluid of women with endometriosis, the proportions of TCRyd cells (p<0.001) and T regulatory cells with the CD4+CD25+CD127low/- phenotype (Treg cells) (p=0.028) were lower than in controls. Compared with peripheral blood, there was a decrease in the proportion of cytotoxic CD3-CD56+CD16+-NK cells (p<0.001), an increase in the proportion of regulatory CD56bnghtCD16dim-NK cells (p=0.005). Compared to the control, the proportion of Treg cells in peripheral blood was also reduced (p=0.028). Besides, the neutrophil-lymphocyte index (p=0.022) was increased. There was a reduction in the proportion of CD14highCD16low -classical monocytes (p=0.017), while the proportion of non-classical was increased (p= 0.009). Six months after surgery, the total leukocyte count, neutrophil-lymphocyte index, and the proportion of Treg cells in the peripheral blood significantly differ from the baseline values (p=0.008, p=0.017, and p=0.001, respectively), not differing from the control values. Conclusion: The imbalance between regulatory and cytotoxic subpopulations is more pronounced at the local than at the systemic level. Nevertheless, after combination therapy, including surgery, there was a noticeable tendency towards normalization of the altered peripheral blood parameters against the background of an improvement in clinical symptoms, particularly in relieving the pain.

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About the authors

Tatiana D. Korotkova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: t_korotkova@oparina4.ru
Obstetrician-Gynecologist, PhD. Student at the Department of Gynecology

Lubov V. Krechetova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: l_krechetova@oparina4.ru
Dr. Med. Sci., Head of the Laboratory of Clinical Immunology

Eugenia V. Inviyaeva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_inviyaeva@oparina4.ru
Ph.D. (bio.sci.), Senior Researcher at the Laboratory of Clinical Immunology

Valentina V. Vtorushina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_vtorushina@oparina4.ru
Ph.D., Immunologist at the Laboratory of Clinical Immunology

Ludmila V. Vanko

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: lvanko@oparina4.ru
Dr. Med. Sci., Professor, Leading Researcher at the Laboratory of Clinical Immunology

Leyla V. Adamyan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: adamyanleila@gmail.com
Dr. Med. Sci., Academician of the RAS, Professor, Head of the Department of Gynecology, Deputy Director for Science

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